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Literature summary extracted from

  • Rueffer, M.; Nagakura, N.; Zenk, M.H.
    Partial purification and properties of S-adenosylmethionine:(R),(S)-norlaudanosoline-6-O-methyltransferase from Argemone platyceras cell culture (1983), J. Med. Plant Res., 49, 131-137.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.128 complete loss of activity after freezing in 30% glycerol solution Argemone platyceras

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.128 Co2+
-
 Argemone platyceras
2.1.1.128 Cu2+
-
 Argemone platyceras
2.1.1.128 Fe2+
-
 Argemone platyceras
2.1.1.128 Hg2+
-
 Argemone platyceras
2.1.1.128 iodobenzoate
-
 Argemone platyceras
2.1.1.128 Mn2+
-
 Argemone platyceras
2.1.1.128 Ni2+
-
 Argemone platyceras
2.1.1.128 PCMB
-
 Argemone platyceras
2.1.1.128 S-adenosyl-L-homocysteine
-
 Argemone platyceras
2.1.1.128 Sn2+
-
 Argemone platyceras
2.1.1.128 Zn2+
-
 Argemone platyceras

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.128 0.05
-
 S-adenosylmethionine with (S)-norlaudanosoline as cosubstrate Argemone platyceras
2.1.1.128 0.2
-
 (R,S)-norlaudanosoline
-
 Argemone platyceras
2.1.1.128 0.3
-
 (R,S)-laudanosoline
-
 Argemone platyceras
2.1.1.128 1.1
-
 (R,S)-4'-O-methylnorlaudanosoline
-
 Argemone platyceras

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.128 47000
-
 gel filtration Argemone platyceras

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.128 Argemone platyceras
-
-
-
2.1.1.128 Papaver somniferum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.128 partial Argemone platyceras

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.128 cell culture
-
 Papaver somniferum
-
2.1.1.128 cell culture
-
 Argemone platyceras
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.128 4°C, 50% loss of activity after 4 weeks Argemone platyceras

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.128 S-Adenosyl-L-methionine + (R)-norlaudanosoline 92% of the activity with (S)-norlaudanosoline Argemone platyceras S-Adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline in the ratio 8:2 ?
2.1.1.128 S-Adenosyl-L-methionine + (R,S)-2,3-dihydroxy-9,10-dimethoxytetrahydroprotoberberine 5% of the activity with (S)-norlaudanosoline Argemone platyceras S-Adenosyl-L-homocysteine + ?
-
 ?
2.1.1.128 S-Adenosyl-L-methionine + (R,S)-4'-O-methylnorlaudanosoline 34% of the activity with (S)-norlaudanosoline Argemone platyceras S-Adenosyl-L-homocysteine + norprotosinomenine
-
 ?
2.1.1.128 S-Adenosyl-L-methionine + (R,S)-5'-O-methylnorlaudanosoline 81% of the activity with (S)-norlaudanosoline Argemone platyceras S-Adenosyl-L-homocysteine + 6-O-methyllaudanosoline
-
 ?
2.1.1.128 S-adenosyl-L-methionine + (R,S)-norlaudanosoline
-
 Argemone platyceras S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline
-
 ?
2.1.1.128 S-adenosyl-L-methionine + (S)-norlaudanosoline
-
 Argemone platyceras S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline in the ratio 8:2 ?
2.1.1.128 S-Adenosyl-L-methionine + (S)-scoulerine 1% of the activity with (S)-norlaudanosoline Argemone platyceras S-Adenosyl-L-homocysteine + jatrorrhizine
-
 ?
2.1.1.128 S-Adenosyl-L-methionine + 2,3-dihydroxy-9,10-dimethoxyprotoberberine 7% of the activity with (S)-norlaudanosoline Argemone platyceras S-Adenosyl-L-homocysteine + tetrahydrojatrorrhizine
-
 ?
2.1.1.128 S-Adenosyl-L-methionine + laudanosoline 79% of the activity with (S)-norlaudanosoline Argemone platyceras S-Adenosyl-L-homocysteine + tetrahydrocolumbamine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.128 35
-
-
 Argemone platyceras

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.128 30
-
 half-life: 8 h Argemone platyceras

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.128 7.5
-
-
 Argemone platyceras