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Literature summary extracted from

  • Nour, J.M.; Rabinowitz, J.C.
    Isolation, characterization, and structural organization of 10-formyltetrahydrofolate synthetase from spinach leaves (1991), J. Biol. Chem., 266, 18363-18369.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.4.3 enzyme is unstable in phosphate buffers in the absence of high concentrations of salt Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.3 Ca2+ in presence of Mg2+ Spinacia oleracea
6.3.4.3 Mn2+ in presence of Mg2+ Spinacia oleracea
6.3.4.3 Zn2+ in presence of Mg2+ Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.3 Ca2+ the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Spinacia oleracea
6.3.4.3 Ca2+ Km for CaCl2: 0.21 mM Spinacia oleracea
6.3.4.3 Cs+ activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ Spinacia oleracea
6.3.4.3 divalent metal ion the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Spinacia oleracea
6.3.4.3 K+ activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ Spinacia oleracea
6.3.4.3 Mg2+ the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Spinacia oleracea
6.3.4.3 Mg2+ Km for MgCl2: 0.44 mM Spinacia oleracea
6.3.4.3 Mn2+ the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ Spinacia oleracea
6.3.4.3 Mn2+ Km for MnCl2: 0.23 mM Spinacia oleracea
6.3.4.3 monovalent cations activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+,NH4+, Na+,Cs+ Spinacia oleracea
6.3.4.3 Na+ activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ Spinacia oleracea
6.3.4.3 NH4+ activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ Spinacia oleracea
6.3.4.3 Rb+ activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.3 67000
-
 2 * 67000, SDS-PAGE Spinacia oleracea
6.3.4.3 130000
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate Spinacia oleracea enzyme of one-carbon metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.3 Spinacia oleracea
-
 spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.3
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.3 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.3 202
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Spinacia oleracea ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate enzyme of one-carbon metabolism Spinacia oleracea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.3 dimer 2 * 67000, SDS-PAGE Spinacia oleracea