Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Alonso, M.J.; De Arriaga, D.; Soler, J.
    Carbamoyl-phosphate synthase in Phycomyces blakesleeanus (1988), Biochim. Biophys. Acta, 955, 175-186.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.16 acetylglutamate not required as activator Phycomyces blakesleeanus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.16 NEM
-
 Phycomyces blakesleeanus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.16 0.93
-
 ATP
-
 Phycomyces blakesleeanus
6.3.4.16 6.34
-
 NH4+
-
 Phycomyces blakesleeanus
6.3.4.16 10.5
-
 HCO3-
-
 Phycomyces blakesleeanus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.16 Mg2+ free Mg2+ required for maximal activity Phycomyces blakesleeanus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.16 55000
-
 1 * 130000 + 1 * 55000, SDS-PAGE Phycomyces blakesleeanus
6.3.4.16 130000
-
 1 * 130000 + 1 * 55000, SDS-PAGE Phycomyces blakesleeanus
6.3.4.16 188000
-
 gel filtration Phycomyces blakesleeanus

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.16 Phycomyces blakesleeanus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.16
-
 Phycomyces blakesleeanus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.16 mycelium
-
 Phycomyces blakesleeanus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.16 0.87
-
-
 Phycomyces blakesleeanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.16 2 ATP + NH4+ + HCO3-
-
 Phycomyces blakesleeanus 2 ADP + phosphate + carbamoyl phosphate
-
 ?
6.3.4.16 2 ATP + NH4+ + HCO3- ATP in form of MgATP2- Phycomyces blakesleeanus 2 ADP + phosphate + carbamoyl phosphate
-
 ?
6.3.4.16 ATP + NH4+ + CO2 + H2O no activity with Gln Phycomyces blakesleeanus ADP + phosphate + carbamoylphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.16 dimer 1 * 130000 + 1 * 55000, SDS-PAGE Phycomyces blakesleeanus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.4.16 38.5
-
 transition temperature Phycomyces blakesleeanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.16 7.4 7.8
-
 Phycomyces blakesleeanus