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Literature summary extracted from

  • Lusty, C.J.
    Carbamoylphosphate synthetase I of rat-liver mitochondria. Purification, properties, and polypeptide molecular weight (1978), Eur. J. Biochem., 85, 373-383.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.16 acetylglutamate activates Rattus norvegicus
6.3.4.16 acetylglutamate Km: 0.1 mM Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.16 2-Amino-4-oxo-5-chloropentanoic acid
-
 Rattus norvegicus
6.3.4.16 Mn2+ activates Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.16 1
-
 ATP MgATP2- Rattus norvegicus
6.3.4.16 2
-
 NH4+
-
 Rattus norvegicus
6.3.4.16 5
-
 HCO3-
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.4.16 mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.16 Co2+ activates Rattus norvegicus
6.3.4.16 Mg2+ Mg2+ and Mn2+ are equally effective activators in the direction of carbamoyl phosphate synthesis, Km: 0.17 mM Rattus norvegicus
6.3.4.16 Mn2+ Mg2+ and Mn2+ are equally effective activators in the direction of carbamoyl phosphate synthesis. Excess Mn2+ inhibits Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.16 158000
-
 1 * 158000, sedimentation equilibrium in 6 M guanidine hydrochloride containing DTT, predominantly exists in the monomeric form Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.16 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.16 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.16 2 ATP + NH4+ + HCO3-
-
 Rattus norvegicus 2 ADP + phosphate + carbamoyl phosphate
-
 ?
6.3.4.16 2 ATP + NH4+ + HCO3- ATP in form of MnATP2- Rattus norvegicus 2 ADP + phosphate + carbamoyl phosphate
-
 ?
6.3.4.16 ATP + NH4+ + CO2 + H2O no activity with Gln Rattus norvegicus ADP + phosphate + carbamoylphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.16 monomer 1 * 158000, sedimentation equilibrium in 6 M guanidine hydrochloride containing DTT, predominantly exists in the monomeric form Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.16 6.8 7.6
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.4.16 6.3 8 50% of maximal activity at pH 6.3 and 8.0 Rattus norvegicus