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Literature summary extracted from

  • Pierson, D.L.; Brien, J.M.
    Human carbamylphosphate synthetase I. Stabilization, purification, and partial characterization of the enzyme from human liver (1980), J. Biol. Chem., 255, 7891-7895.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.16 acetylglutamate activates Homo sapiens
6.3.4.16 acetylglutamate Km: 0.1 mM Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.16 Mg2+ above 20 mM Homo sapiens
6.3.4.16 Mn2+ at or above 5 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.16 0.8
-
 NH4+
-
 Homo sapiens
6.3.4.16 1.1
-
 ATP
-
 Homo sapiens
6.3.4.16 6.7
-
 HCO3-
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.16 Mg2+ requirement for free Mg2+ in addition to the MgATP2- complex, inhibits above 20 mM Homo sapiens
6.3.4.16 Mn2+ can substitute for Mg2+ in activation, more effective on a molar basis, free Mn2+ at 5 mM and above inhibits Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.16 165000
-
 1 * 165000, SDS-PAGE Homo sapiens
6.3.4.16 190000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.16 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.16
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.16 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.16 1.5
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.16 2 ATP + NH4+ + HCO3-
-
 Homo sapiens 2 ADP + phosphate + carbamoyl phosphate
-
 ?
6.3.4.16 2 ATP + NH4+ + HCO3- ATP in form of MgATP2- Homo sapiens 2 ADP + phosphate + carbamoyl phosphate
-
 ?
6.3.4.16 ATP + NH4+ + CO2 + H2O
-
 Homo sapiens ADP + phosphate + carbamoylphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.16 monomer 1 * 165000, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.16 7.8
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.4.16 7 8.5 50% of maximal activity at pH 7.0 and 8.5 Homo sapiens