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Literature summary extracted from

  • Rubio, V.; Ramponi, G.; Grisolia, S.
    Carbamoyl phosphate synthetase I of human liver. Purification, some properties and immunological cross-reactivity with the rat liver enzyme (1981), Biochim. Biophys. Acta, 659, 150-160.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.16 acetylglutamate activates Homo sapiens
6.3.4.16 acetylglutamate Km: 0.15 mM Homo sapiens
6.3.4.16 N-carbamoylglutamate effective activator, Km: 2.0 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.16 0.26
-
ATP
-
Homo sapiens
6.3.4.16 1.3
-
NH4+
-
Homo sapiens
6.3.4.16 2.2
-
ATP HCO3- Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.16 160000
-
1 * 160000, SDS-PAGE in presence of a reducing agent Homo sapiens
6.3.4.16 165000 178000 gel filtration, density gradient centrifugation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.16 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.16
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.16 liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.16 1.5
-
-
Homo sapiens

Storage Stability

EC Number Storage Stability Organism
6.3.4.16 4°C, stable for at least 1 month Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.16 2 ATP + NH4+ + HCO3- ATP in form of CoATP2- Homo sapiens 2 ADP + phosphate + carbamoyl phosphate
-
?
6.3.4.16 ATP + NH4+ + CO2 + H2O no activity with Gln Homo sapiens ADP + phosphate + carbamoylphosphate
-
?

Subunits

EC Number Subunits Comment Organism
6.3.4.16 monomer 1 * 160000, SDS-PAGE in presence of a reducing agent Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.4.16 37
-
4 h, 30% loss of activity Homo sapiens
6.3.4.16 46
-
5 min, 25% loss of activity Homo sapiens
6.3.4.16 55
-
5 min, 80% loss of activity Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.16 additional information
-
additional information
-
Homo sapiens