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Literature summary extracted from

  • Raijman, L.; Jones, M.E.
    Purification, composition, and some properties of rat liver carbamyl phosphate synthetase (ammonia) (1976), Arch. Biochem. Biophys., 175, 270-278.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.4.16 glycerol and DTT stabilize during purification Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.4.16 mitochondrial matrix constitutes 22-26% of the total matrix protein Rattus norvegicus 5759
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.16 120000
-
 2 * 120000, SDS-PAGE Rattus norvegicus
6.3.4.16 160000 251000 sedimentation velocity measurement, gel filtration, MW depends on protein concentration and composition of solvent Rattus norvegicus
6.3.4.16 222000
-
 glycerol or sucrose density gradient centrifugation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.16 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.16
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.16 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.16 1.87
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
6.3.4.16 4°C, 20% v/v glycerol, 0.1 or 2 mM DTT, 48 h, stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.16 ATP + NH4+ + CO2 + H2O
-
 Rattus norvegicus ADP + phosphate + carbamoylphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.16 dimer 2 * 120000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.4.16 23
-
 24 h, 20% v/v glycerol, 2 mM DTT, stable Rattus norvegicus
6.3.4.16 23
-
 24 h, 20% v/v glycerol, 2 mM DTT, 13% loss of activity Rattus norvegicus