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Literature summary extracted from

  • Guchhait, R.B.; Polakis, S.E.; Dimroth, P.; Stoll, E.; Moss, J.; Lane, M.D.
    Acetyl coenzyme A carboxylase system of Escherichia coli. Purification and properties of the biotin carboxylase, carboxyltransferase, and carboxyl carrier protein components (1974), J. Biol. Chem., 249, 6633-6645.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.14 ethanol maximal activation, 10fold, at 15% v/v. Inactivation at 20% v/v Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.14
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.14 Co2+ above 2 mM Escherichia coli
6.3.4.14 ethanol maximal activation, 10fold, at 15% v/v. Inactivation at 20% v/v Escherichia coli
6.3.4.14 Mn2+ above 2 mM Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.14 Co2+ divalent metal ion required, Mg2+, Mn2+ or Co2+ Escherichia coli
6.3.4.14 Mg2+ divalent metal ion required, Mg2+, Mn2+ or Co2+ Escherichia coli
6.3.4.14 Mn2+ divalent metal ion required, Mg2+, Mn2+ or Co2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.14 50000
-
 2 * 50000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.14 Escherichia coli
-
 B
-
6.3.4.14 Escherichia coli B / ATCC 11303
-
 B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.14
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.14 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.14 ATP + biotin-carboxyl-carrier protein + HCO3-
-
 Escherichia coli ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?
6.3.4.14 ATP + biotin-carboxyl-carrier protein + HCO3-
-
 Escherichia coli B / ATCC 11303 ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?
6.3.4.14 ATP + D-(+)-biotin + HCO3- L-biotin is completely inactive Escherichia coli ADP + phosphate + carboxybiotin
-
 ?
6.3.4.14 ATP + D-(+)-biotin + HCO3- L-biotin is completely inactive Escherichia coli B / ATCC 11303 ADP + phosphate + carboxybiotin
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.14 dimer 2 * 50000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.14 6.5
-
 Mn2+-activated reaction Escherichia coli
6.3.4.14 8
-
 Mg2+-activated reaction Escherichia coli