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Literature summary extracted from

  • Tipton, P.A.; Cleland, W.W.
    Catalytic mechanism of biotin carboxylase: steady-state kinetic investigation (1988), Biochemistry, 27, 4317-4325.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.14 2-Pyridyl disulfide
-
 Escherichia coli
6.3.4.14 NEM
-
 Escherichia coli
6.3.4.14 Thionucleotides
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.14 0.00126
-
 ADP
-
 Escherichia coli
6.3.4.14 0.0025
-
 ATP
-
 Escherichia coli
6.3.4.14 0.0076
-
 ATP
-
 Escherichia coli
6.3.4.14 0.0398
-
 ADP
-
 Escherichia coli
6.3.4.14 0.0546
-
 ATP
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.14 Co2+ divalent metal ion required, Mg2+, Mn2+ or Co2+ Escherichia coli
6.3.4.14 Mg2+ divalent metal ion required, Mg2+, Mn2+ or Co2+ Escherichia coli
6.3.4.14 Mg2+ Km: 0.57 mM Escherichia coli
6.3.4.14 Mn2+ divalent metal ion required, Mg2+, Mn2+ or Co2+ Escherichia coli
6.3.4.14 Mn2+ Km: 0.11 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.14 51000
-
 2 * 51000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.14 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.14
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.14 ATP + [biotin carboxyl-carrier protein]-biotin-N6-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine carboxylation proceeds via a carboxyphosphate intermediate Escherichia coli
a
6.3.4.14 ATP + [biotin carboxyl-carrier protein]-biotin-N6-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine MgATP2- and HCO3- add to the enzyme randomly, followed by addition of biotin. Both HCO3- and MgATP2- add in rapid equilibrium Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.14 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.14 ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine ADP in form of MgADP- Escherichia coli ATP + [biotin carboxyl-carrier protein]-biotin-N6-L-lysine + hydrogencarbonate
-
 r
6.3.4.14 ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine ADP in form of MnADP- Escherichia coli ATP + [biotin carboxyl-carrier protein]-biotin-N6-L-lysine + hydrogencarbonate
-
 r
6.3.4.14 ATP + biotin-carboxyl-carrier protein + HCO3-
-
 Escherichia coli ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?
6.3.4.14 ATP + [biotin carboxyl-carrier protein]-biotin-N6-L-lysine + hydrogencarbonate ATP in form of MnATP2- Escherichia coli ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine
-
 r
6.3.4.14 ATP + [biotin carboxyl-carrier protein]-biotin-N6-L-lysine + hydrogencarbonate ATP in form of MgATP2- Escherichia coli ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine
-
 r
6.3.4.14 ATP + [biotin carboxyl-carrier protein]-biotin-N6-L-lysine + hydrogencarbonate ATP in form of CoATP2- Escherichia coli ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N6-L-lysine
-
 r
6.3.4.14 additional information enzyme catalyzes ATP-hydrolysis in absence of biotin Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.14 dimer 2 * 51000, SDS-PAGE Escherichia coli