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Literature summary extracted from

  • Niyomporn, B.; Dahl, J.L.; Strominger, J.L.
    Biosynthesis of the peptidoglycan of bacterial cell walls. IX. Purification and properties of glycyl transfer ribonucleic acid synthetase from Staphylococcus aureus (1968), J. Biol. Chem., 243, 773-778.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.14 AMP
-
 Staphylococcus aureus
6.1.1.14 citrate
-
 Staphylococcus aureus
6.1.1.14 KCl inhibits acylation of yeast tRNA, little effect on acylation of homologous tRNAGly Staphylococcus aureus
6.1.1.14 p-chloromercuriphenylsulfonic acid activity is not restored by treatment with 2-mercaptoethanol Staphylococcus aureus
6.1.1.14 phosphate
-
 Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.14 0.001
-
 tRNAGly Gly, , ATP-diphosphate exchange Staphylococcus aureus
6.1.1.14 0.19
-
 ATP ATP-diphosphate exchange Staphylococcus aureus
6.1.1.14 0.22
-
 ATP glycyl-tRNA synthesis Staphylococcus aureus
6.1.1.14 0.67
-
 Gly glycyl-tRNA synthesis Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.14 Co2+ activates with 5% of the efficiency of ATP Staphylococcus aureus
6.1.1.14 Li+ slightly stimulates in presence of Mg2+ Staphylococcus aureus
6.1.1.14 Mg2+ required Staphylococcus aureus
6.1.1.14 Mg2+ optimal Mg2+/ATP ratio is 5:1 Staphylococcus aureus
6.1.1.14 Mn2+ inhibition in presence of Mg2+ Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.14 ATP + glycine + tRNAGly Staphylococcus aureus protein synthesis ?
-
 ?
6.1.1.14 ATP + glycine + tRNAGly Staphylococcus aureus H protein synthesis ?
-
 ?
6.1.1.14 additional information Staphylococcus aureus participates in peptidoglycan synthesis ?
-
 ?
6.1.1.14 additional information Staphylococcus aureus H participates in peptidoglycan synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.14 Staphylococcus aureus
-
-
-
6.1.1.14 Staphylococcus aureus H
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.14
-
 Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.14 0.47
-
-
 Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.14 ATP + glycine + tRNAGly replacement of ATP by CTP, UTP, ITP, GTP, or TTP results in a decrease in the reaction rate to less than 7% of that with ATP Staphylococcus aureus AMP + diphosphate + glycyl-tRNAGly
-
 ?
6.1.1.14 ATP + glycine + tRNAGly can also charge the tRNAs of certain other bacteria and yeast Staphylococcus aureus AMP + diphosphate + glycyl-tRNAGly
-
 ?
6.1.1.14 ATP + glycine + tRNAGly replacement of ATP by CTP, UTP, ITP, GTP, or TTP results in a decrease in the reaction rate to less than 7% of that with ATP Staphylococcus aureus H AMP + diphosphate + glycyl-tRNAGly
-
 ?
6.1.1.14 ATP + glycine + tRNAGly can also charge the tRNAs of certain other bacteria and yeast Staphylococcus aureus H AMP + diphosphate + glycyl-tRNAGly
-
 ?
6.1.1.14 ATP + glycine + tRNAGly protein synthesis Staphylococcus aureus ?
-
 ?
6.1.1.14 ATP + glycine + tRNAGly protein synthesis Staphylococcus aureus H ?
-
 ?
6.1.1.14 additional information catalyzes glycine-dependent ATP-diphosphate exchange Staphylococcus aureus ?
-
 ?
6.1.1.14 additional information participates in peptidoglycan synthesis Staphylococcus aureus ?
-
 ?
6.1.1.14 additional information catalyzes glycine-dependent ATP-diphosphate exchange Staphylococcus aureus H ?
-
 ?
6.1.1.14 additional information participates in peptidoglycan synthesis Staphylococcus aureus H ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.14 8 8.5
-
 Staphylococcus aureus