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Literature summary extracted from

  • Siegel, L.; Foote, J.L.; Coon, M.J.
    The enzymatic synthesis of propionyl coenzyme A holocarboxylase from d-biotinyl 5'-adenylate and the apocarboxylase (1965), J. Biol. Chem., 240, 1025-1031.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.4.10 loss of activity upon freezing Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.10 p-chloromercuriphenylsulfonate
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.10 0.0000047
-
 biotin
-
 Oryctolagus cuniculus
6.3.4.10 0.000385
-
 ATP
-
 Oryctolagus cuniculus
6.3.4.10 0.0013
-
 ATP
-
 Oryctolagus cuniculus
6.3.4.10 0.0056
-
 biotinyl-5'-AMP
-
 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.10 Mg2+ required Oryctolagus cuniculus
6.3.4.10 Mg2+ Mg2+ is probably required primarily for the synthesis of biotinyl-AMP from ATP and biotin, but not for the transfer of the biotin moiety from this intermediate to the apocarboxylase Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] Oryctolagus cuniculus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.10 Oryctolagus cuniculus
-
-
-
6.3.4.10 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.10
-
 Oryctolagus cuniculus
6.3.4.10 apocarboxylase Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.10 liver
-
 Rattus norvegicus
-
6.3.4.10 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.10 additional information
-
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
6.3.4.10 0°C, 0.5 M sucrose, stable for about 1 week Oryctolagus cuniculus
6.3.4.10 4°C, stable for several weeks Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Rattus norvegicus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] biotinyl-AMP is an intermediate Oryctolagus cuniculus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Oryctolagus cuniculus ?
-
 ?
6.3.4.10 biotinyl-5'-AMP + apocarboxylase
-
 Oryctolagus cuniculus AMP + holocarboxylase
-
 ?
6.3.4.10 CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 19% of the activity relative to ATP Oryctolagus cuniculus CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 11% of the activity relative to ATP Oryctolagus cuniculus GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.10 UTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 24% of the activity relative to ATP Oryctolagus cuniculus UMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.4.10 53
-
 10-19% loss of activity after 1 min, 73-76% loss of activity after 5 min, 91-93% loss of activity after 15 min Oryctolagus cuniculus
6.3.4.10 53
-
 10% loss of activity after 1 min, 63% loss of activity after 5 min, 91% loss of activity after 15 min Oryctolagus cuniculus