Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lambert, J.M.; Boocock, M.R.; Coggins, J.R.
    The 3-dehydroquinate synthase activity of the pentafunctional arom enzyme complex of Neurospora crassa is Zn2+-dependent (1985), Biochem. J., 226, 817-829.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.4 1,10-phenanthroline
-
 Neurospora crassa
4.2.3.4 bistrispropane
-
 Neurospora crassa
4.2.3.4 Cu2+
-
 Neurospora crassa
4.2.3.4 dithiothreitol
-
 Neurospora crassa
4.2.3.4 EDTA
-
 Neurospora crassa
4.2.3.4 Fe2+
-
 Neurospora crassa
4.2.3.4 NADH
-
 Neurospora crassa
4.2.3.4 Ni2+
-
 Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.4 0.0014
-
 3-deoxy-D-arabino-heptulosonic acid 7-phosphate
-
 Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.4 Zn2+
-
 Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Neurospora crassa
-
-
-
2.5.1.19 Neurospora crassa 74-OR23-1A
-
-
-
4.2.3.4 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19 enzyme is part of multifunctional protein with distinct domains Neurospora crassa
4.2.3.4 arom-multienzyme complex Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.4 additional information
-
-
 Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
2.5.1.19 -20°C, more than a year Neurospora crassa
4.2.3.4 -20°C, more than 1 year Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Neurospora crassa phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Neurospora crassa 74-OR23-1A phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
4.2.3.4 3-deoxy-D-arabino-heptulosonic acid 7-phosphate
-
 Neurospora crassa 3-dehydroquinate + phosphate
-
 ?
4.2.3.4 3-deoxy-D-arabino-heptulosonic acid 7-phosphate
-
 Neurospora crassa 74-OR23-1A 3-dehydroquinate + phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.19 33.6
-
 3-phosphoshikimate cosubstrate phosphoenolpyruvate Neurospora crassa
2.5.1.19 33.6
-
 phosphoenolpyruvate cosubstrate 3-phosphoshikimate Neurospora crassa
4.2.3.4 19
-
 3-deoxy-D-arabino-heptulosonic acid 7-phosphate
-
 Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.3.4 NAD+
-
 Neurospora crassa