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Literature summary extracted from

  • Haire, M.F.; Clark, J.J.; Jones, M.E.E.; Hendil, K.B.; Schwartz, L.M.; Mykles, D.L.
    The multicatalytic proteinase (proteasome) of the hawkmoth, Manduca sexta: Catalytic properties and immunological comparison with the lobster enzyme complex (1995), Arch. Biochem. Biophys., 318, 15-24.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.25.1 SDS stimulates peptidylglutamyl peptide hydrolase, branched-chain amino acid preferring and caseinolytic activities Manduca sexta

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.25.1 3,4-dichloroisocoumarin
-
 Manduca sexta
3.4.25.1 ATP inhibits trypsin-like, peptidylglutamyl peptide hydrolase, and branched-chain amino acid preferring activities Manduca sexta
3.4.25.1 leupeptin inhibits trypsin-like activity Manduca sexta
3.4.25.1 Mg2+ inhibits trypsin like activity Manduca sexta
3.4.25.1 N-ethylmaleimide inhibits trypsin-like and chymotrypsin-like activities Manduca sexta
3.4.25.1 SDS inhibits trypsin-like and chymotrypsin-like activities Manduca sexta

Organism

EC Number Organism UniProt Comment Textmining
3.4.25.1 Manduca sexta
-
 hawkmoth
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.25.1
-
 Manduca sexta

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.25.1 cleavage of peptide bonds with very broad specificity trypsin-like, chymotrypsin-like activities, peptidylglutamyl peptide hydrolase and branched-chain amino acid preferring and caseinolytic activities Manduca sexta
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.25.1 integument
-
 Manduca sexta
-
3.4.25.1 intersegmental muscle
-
 Manduca sexta
-
3.4.25.1 larva
-
 Manduca sexta
-
3.4.25.1 ovary
-
 Manduca sexta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.25.1 0.016
-
-
 Manduca sexta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.25.1 benzyloxycarbonyl-Gly-Gly-Arg-beta-naphthylamide + H2O
-
 Manduca sexta ?
-
 ?
3.4.25.1 benzyloxycarbonyl-Gly-Gly-Leu-p-nitroanilide + H2O
-
 Manduca sexta ?
-
 ?
3.4.25.1 benzyloxycarbonyl-Gly-Pro-Ala-Gly-Gly-p-aminobenzoate + aminopeptidase-N + H2O
-
 Manduca sexta ?
-
 ?
3.4.25.1 benzyloxycarbonyl-Gly-Pro-Ala-Leu-Ala-p-aminobenzoate + aminopeptidase-N + H2O
-
 Manduca sexta ?
-
 ?
3.4.25.1 benzyloxycarbonyl-Leu-Leu-Glu-beta-naphthylamide + H2O
-
 Manduca sexta ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
3.4.25.1 ATP stimulates the caseinolytic activity Manduca sexta