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Literature summary extracted from
- C-terminal specific protein degradation: activity and substrate specificity of the Tsp protease (1995), Protein Sci., 4, 1507-1515.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.102 | 0.035 | - |
Ac-WARAAARAAARBAAB | - |
Escherichia coli |  |
| 3.4.21.102 | 0.05 | - |
Arc repressor | - |
Escherichia coli |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.102 | 75000 | - |
TspH6, enzyme protein with six additional His residues at the C-terminus, calculation from sequence of cDNA | Escherichia coli |
| 3.4.21.102 | 78500 | - |
TspH6, enzyme protein with six additional His residues at the C-terminus, sedimentation equilibrium | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.102 | Protein + H2O | Escherichia coli | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.102 | Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.102 | Ac-WARAAARAAARBAAB + H2O | i.e. peptide BAS9 | Escherichia coli | ? | - |
? | |
| 3.4.21.102 | Ac-WARAAARAAARBGGB + H2O | i.e. peptide BAS10 | Escherichia coli | ? | - |
? | |
| 3.4.21.102 | Arc repressor + H2O | C-terminal sequence: GRIGA, endoproteolyric cleavage | Escherichia coli | ? | - |
? | |
| 3.4.21.102 | Protein + H2O | - |
Escherichia coli | ? | - |
? | |
| 3.4.21.102 | variant of the lambda repressor + H2O | C-terminal sequence: WVAAA, endoproteolytic cleavage | Escherichia coli | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.102 | monomer | sedimentation equilibrium sequence of cDNA | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.102 | 0.19 | - |
Arc repressor | - |
Escherichia coli | |
| 3.4.21.102 | 3.7 | - |
Ac-WARAAARAAARBAAB | - |
Escherichia coli | |
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