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Literature summary extracted from

  • Patterson, E.K.; Gatmaitan, J.S.; Hayman, S.
    Substrate specificity and pH dependence of dipeptidases purified from Escherichia coli B and from mouse ascites tumor cells (1973), Biochemistry, 12, 3701-3709.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.13.18 stable to 18 h dialysis, at 10°C against 0.02 M phosphate buffer, pH 7.3-8.3 Mus musculus
3.4.13.18 stable to 18 h dialysis, at 10°C against 0.02 M phosphate buffer, pH 7.3-8.3 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.18 2-mercaptoethanol
-
 Escherichia coli
3.4.13.18 2-mercaptoethanol
-
 Mus musculus
3.4.13.18 cysteine
-
 Escherichia coli
3.4.13.18 cysteine
-
 Mus musculus
3.4.13.18 D-Leu-Gly competitive inhibitor of L-Ala-Gly hydrolysis Escherichia coli
3.4.13.18 D-Leu-Gly competitive inhibitor of L-Ala-Gly hydrolysis Mus musculus
3.4.13.18 DTT
-
 Escherichia coli
3.4.13.18 DTT
-
 Mus musculus
3.4.13.18 iodoacetate not Escherichia coli
3.4.13.18 iodoacetate not Mus musculus
3.4.13.18 Iodosobenzoate
-
 Escherichia coli
3.4.13.18 Iodosobenzoate
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.18 0.25
-
 L-Leu-L-Phe
-
 Escherichia coli
3.4.13.18 0.25
-
 Leu-Leu tumor enzyme Mus musculus
3.4.13.18 0.25
-
 Leu-Leu tumor enzyme Escherichia coli
3.4.13.18 0.28
-
 Ala-Leu tumor enzyme Mus musculus
3.4.13.18 0.29
-
 Met-Leu
-
 Escherichia coli
3.4.13.18 0.33
-
 Leu-Gly tumor enzyme Mus musculus
3.4.13.18 2
-
 Gly-L-Lys
-
 Escherichia coli
3.4.13.18 29
-
 Gly-L-Asn tumor enzyme Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.18 85000
-
 gel filtration Mus musculus
3.4.13.18 100000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.18 Escherichia coli
-
-
-
3.4.13.18 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.18
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.13.18 ascites tumor cell
-
 Mus musculus
-

Storage Stability

EC Number Storage Stability Organism
3.4.13.18 -30°C, 0.02 M potassium phosphate, pH 8.2, 0.25 M sucrose, stable Mus musculus
3.4.13.18 -30°C, 0.02 M potassium phosphate, pH 8.2, 0.25 M sucrose, stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.18 Ala-Ile + H2O best substrate for the tumor enzyme Mus musculus Ala + Ile
-
 ?
3.4.13.18 Ala-Leu + H2O
-
 Mus musculus Ala + Leu
-
 ?
3.4.13.18 Gly-L-Asn + H2O
-
 Mus musculus Gly + L-Asn
-
 ?
3.4.13.18 Gly-L-Lys + H2O
-
 Escherichia coli Gly + L-Lys
-
 ?
3.4.13.18 L-Ala-Gly + H2O
-
 Mus musculus L-Ala + Gly
-
 ?
3.4.13.18 L-Ala-Gly + H2O
-
 Escherichia coli L-Ala + Gly
-
 ?
3.4.13.18 L-Leu-L-Phe + H2O
-
 Escherichia coli L-Leu + L-Phe
-
 ?
3.4.13.18 Leu-Gly + H2O
-
 Mus musculus Leu + Gly
-
 ?
3.4.13.18 Leu-Gly + H2O
-
 Escherichia coli Leu + Gly
-
 ?
3.4.13.18 Leu-Leu + H2O
-
 Mus musculus Leu + Leu
-
 ?
3.4.13.18 Leu-Leu + H2O
-
 Escherichia coli Leu + Leu
-
 ?
3.4.13.18 Met-Ala + H2O best substrate for E. coli enzyme Escherichia coli Met + Ala
-
 ?
3.4.13.18 Met-Leu + H2O
-
 Escherichia coli Met + Leu
-
 ?
3.4.13.18 additional information specificity overview Mus musculus ?
-
 ?
3.4.13.18 additional information specificity overview Escherichia coli ?
-
 ?
3.4.13.18 additional information the tumor enzyme has the highest maximal velocities with substrates containing uncharged bulky C-terminal and small, uncharged N-terminal side chains, the bacterial enzyme hydrolyzes most rapidly substrates with long or flat N-terminal R-groups and small or positively charged C-terminal groups Mus musculus ?
-
 ?
3.4.13.18 additional information the tumor enzyme has the highest maximal velocities with substrates containing uncharged bulky C-terminal and small, uncharged N-terminal side chains, the bacterial enzyme hydrolyzes most rapidly substrates with long or flat N-terminal R-groups and small or positively charged C-terminal groups Escherichia coli ?
-
 ?
3.4.13.18 additional information only unsubstituted L-alpha-dipeptides Mus musculus ?
-
 ?
3.4.13.18 additional information only unsubstituted L-alpha-dipeptides Escherichia coli ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.18 8
-
-
 Escherichia coli
3.4.13.18 8.3
-
 ascites tumor enzyme Mus musculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.13.18 6
-
 6 h, 80% loss of activity, ascites tumor enzyme Mus musculus
3.4.13.18 6.2 9 6 h stable Escherichia coli
3.4.13.18 6.7
-
 6 h, 80% loss of activity, mouse ascites tumor enzyme Mus musculus
3.4.13.18 7
-
 6 h, 25-30% loss of activity, mouse ascites tumor enzyme Mus musculus
3.4.13.18 8.1 8.3 6 h, stable Mus musculus
3.4.13.18 8.9
-
 6 h, 25-30% loss of activity, mouse ascites tumor enzyme Mus musculus