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Literature summary extracted from

  • Wilk, S.; Wilk, E.; Magnusson, R.P.
    Purification, characterization and cloning of a cytosolic aspartyl aminopeptidase (1998), J. Biol. Chem., 273, 15961-15970.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.21 bacitracin activating at 0.2 mM Oryctolagus cuniculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.21 expression in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.21 1,10-phenanthroline after preincubation total loss of activity Oryctolagus cuniculus
3.4.11.21 dithiothreitol 88% inhibition at 1 mM Oryctolagus cuniculus
3.4.11.21 p-chloromercuribenzoate
-
Oryctolagus cuniculus
3.4.11.21 Zn2+ 50% inhibition at 0.4 M, after preincubation total inhibition Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.21 0.05 2.8 Asp-Xaa-Xaa-Xaa
-
Oryctolagus cuniculus
3.4.11.21 0.25
-
angiotensin II
-
Oryctolagus cuniculus
3.4.11.21 2
-
Asp-Ala-Pro-sulfamethoxazole
-
Oryctolagus cuniculus
3.4.11.21 2
-
Asp-Ala-Pro-naphthylamide
-
Oryctolagus cuniculus
3.4.11.21 2.3
-
Asp-Ala-Pro-sulfamethoxazole overexpressed enzyme Homo sapiens
3.4.11.21 8.4
-
Glu-Ala-Pro-naphthylamide
-
Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.21 cytosol
-
Oryctolagus cuniculus 5829
-
3.4.11.21 cytosol
-
Rattus sp. 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.21 55000
-
SDS-PAGE Oryctolagus cuniculus
3.4.11.21 440000
-
native gel filtration Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.21 angiotensin + H2O Homo sapiens intracellular protein and peptide metabolism Asp + des-Asp-angiotensin
-
?
3.4.11.21 angiotensin + H2O Oryctolagus cuniculus intracellular protein and peptide metabolism Asp + des-Asp-angiotensin
-
?
3.4.11.21 angiotensin + H2O Rattus sp. intracellular protein and peptide metabolism Asp + des-Asp-angiotensin
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.21 Homo sapiens
-
-
-
3.4.11.21 Oryctolagus cuniculus
-
rabbit
-
3.4.11.21 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.21 722fold near homogeneity by conventional chromatography techniques Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.21 brain
-
Oryctolagus cuniculus
-
3.4.11.21 brain
-
Rattus sp.
-
3.4.11.21 heart
-
Rattus sp.
-
3.4.11.21 intestine
-
Rattus sp.
-
3.4.11.21 kidney
-
Rattus sp.
-
3.4.11.21 liver
-
Rattus sp.
-
3.4.11.21 lung
-
Rattus sp.
-
3.4.11.21 spleen
-
Rattus sp.
-
3.4.11.21 testis
-
Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.21 0.013 0.058
-
Rattus sp.
3.4.11.21 0.272 0.332 overexpression in Escherichia coli Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.21 angiotensin + H2O intracellular protein and peptide metabolism Homo sapiens Asp + des-Asp-angiotensin
-
?
3.4.11.21 angiotensin + H2O intracellular protein and peptide metabolism Oryctolagus cuniculus Asp + des-Asp-angiotensin
-
?
3.4.11.21 angiotensin + H2O intracellular protein and peptide metabolism Rattus sp. Asp + des-Asp-angiotensin
-
?
3.4.11.21 angiotensin II + H2O octapeptides preferred to tetrapeptides Oryctolagus cuniculus Asp + angiotensin III
-
?
3.4.11.21 Asp-Ala-Pro-2-naphthylamide + H2O preferring acidic amino acids favouring aspartyl residues, requires a free alpha-amino group Oryctolagus cuniculus Asp + Ala-Pro-2-naphthylamide
-
?
3.4.11.21 Asp-Ala-Pro-sulfamethoxazole + H2O
-
Homo sapiens Asp + Ala-Pro-sulfamethoxazole
-
?
3.4.11.21 Asp-Ala-Pro-sulfamethoxazole + H2O
-
Oryctolagus cuniculus Asp + Ala-Pro-sulfamethoxazole
-
?
3.4.11.21 Asp-Xaa-Xaa-Xaa + H2O neutral or hydrophobic amino acids in P1 position preferred Oryctolagus cuniculus Asp + Xaa-Xaa-Xaa
-
?
3.4.11.21 Glu-Ala-Pro-naphthylamide + H2O
-
Oryctolagus cuniculus Glu + Ala-Pro-naphthylamide
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.21 7 7.5
-
Oryctolagus cuniculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.21 0.4
-
Zn2+ after preincubation total inhibition Oryctolagus cuniculus