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Literature summary extracted from

  • Guranowski, A.; Schneider, Z.
    Purification and characterization of adenosine nucleosidase from barley leaves (1977), Biochim. Biophys. Acta, 482, 145-158.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.2.7 presence of salt essential Hordeum vulgare
3.2.2.7 stable against repeated freezing and thawing cycles Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.7 2'-deoxyadenosine Ki: 0.05 mM Hordeum vulgare
3.2.2.7 3'-deoxyadenosine Ki: four times weaker than for 2'-deoxyadenosine Hordeum vulgare
3.2.2.7 adenine strong competitive inhibitor Hordeum vulgare
3.2.2.7 cytokinin plant hormones, inhibition less strong than for adenine Hordeum vulgare
3.2.2.7 dithiothreitol 60% inhibition if enzyme is preincubated with 10 mM Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.7 0.001
-
adenosine in MES buffer, pH 5.4 Hordeum vulgare
3.2.2.7 0.12
-
2'-deoxyadenosine in MES buffer, pH 5.4 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.7 33000
-
alpha2, 2 * 33000, non-reducing SDS-PAGE Hordeum vulgare
3.2.2.7 66000
-
gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.7 2'-deoxyadenosine + H2O Hordeum vulgare 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside 6-aminopurine + 2-deoxy-D-ribofuranose
-
?
3.2.2.7 adenosine + H2O Hordeum vulgare 6-aminopurine-9-beta-D-ribofuranoside 6-aminopurine + D-ribofuranose
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.7 Hordeum vulgare
-
barley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.7 to homogeneity, chromatography steps Hordeum vulgare

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.2.7 adenosine + H2O = D-ribose + adenine also acts on adenosine N-oxide Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.7 leaf
-
Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.7 30.5
-
-
Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
3.2.2.7 storage in dried state in Sephadex G-25 beads remains stability for several months Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.7 2'-deoxyadenosine + H2O 6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside Hordeum vulgare 6-aminopurine + 2-deoxy-D-ribofuranose
-
?
3.2.2.7 6-aminopurine-9-beta-D-ribofuranosyl-N1-oxide + H2O same hydrolysis rate than for adenosine or 2'-deoxyadenosine Hordeum vulgare 6-aminopurine-N1-oxide + D-ribofuranose
-
?
3.2.2.7 adenosine + H2O 6-aminopurine-9-beta-D-ribofuranoside Hordeum vulgare 6-aminopurine + D-ribofuranose
-
?
3.2.2.7 purine riboside + H2O low activity Hordeum vulgare purine + D-ribose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.2.7 dimer alpha2, 2 * 33000, non-reducing SDS-PAGE Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.7 58
-
-
Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.7 4.7
-
in 40 mM citrate buffer Hordeum vulgare
3.2.2.7 5.4
-
in 50 mM MES/KOH buffer Hordeum vulgare

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.2.7 0.05
-
2'-deoxyadenosine
-
Hordeum vulgare