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Literature summary extracted from

  • Leon, O.; Quintana, L.; Peruzzo, P.; Slebe, J.C.
    Purification and properties of an extracellular agarase from Alteromonas sp. strain C-1 (1992), Appl. Environ. Microbiol., 58, 4060-4063.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.81 KCl 50% inhibition at 0.1 M Alteromonas sp.
3.2.1.81 NaCl 50% inhibition at 0.1 M Alteromonas sp.
3.2.1.81 NH4Cl 50% inhibition at 0.1 M Alteromonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.81 1.7
-
neoagarohexaose
-
Alteromonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.81 extracellular
-
Alteromonas sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.81 52000
-
gel filtration Alteromonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.81 agarose + H2O Alteromonas sp.
-
neoagarotetraose
-
ir

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.81 Alteromonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.81
-
Alteromonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.81 234
-
-
Alteromonas sp.

Storage Stability

EC Number Storage Stability Organism
3.2.1.81 4°C, Tris-HCl with 0.4 M NaCl, pH 7.5, more than 6 months Alteromonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.81 agarose + H2O
-
Alteromonas sp. neoagarotetraose
-
ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.81 30
-
inhibition above 30°C, after 20 min 35°C 50% of activity Alteromonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.81 6.5
-
-
Alteromonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.81 0.1
-
NaCl
-
Alteromonas sp.
3.2.1.81 0.1
-
KCl
-
Alteromonas sp.
3.2.1.81 0.1
-
NH4Cl
-
Alteromonas sp.