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Literature summary extracted from
- Purification, characterization, and cDNA structure of isoamylase from developing endosperm of rice (1999), Planta, 208, 283-293.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.68 | isoamylase gene is a single copy in rice genome, located on chromsome 8 | Oryza sativa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.68 | (NH4)2Mo2O7 | 1 mM 22% inhibition, 10 mM 100% inhibition | Oryza sativa |  |
| 3.2.1.68 | beta-cyclodextrin | 29%, slightly inhibitory at 10 mM | Oryza sativa | |
| 3.2.1.68 | CuCl2 | 0.1 mM reduce activity to 3% | Oryza sativa | |
| 3.2.1.68 | HgCl2 | 1 mM, complete inhibition | Oryza sativa | |
| 3.2.1.68 | iodoacetate | 21%, slight inhibition | Oryza sativa | |
| 3.2.1.68 | p-chloromercuribenzoate | - |
Oryza sativa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.68 | additional information | - |
additional information | amylopectin 7.2 mg/ml-1, phytoglycogen 11.9 mg/ml-1, beta-limit dextrin 13.1 mg/ml-1, glycogen 21.8 mg/ml-1 | Oryza sativa |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.68 | 83000 | - |
- |
Oryza sativa |
| 3.2.1.68 | 340000 | - |
native enzyme, gel filtration, TSKgel G3000SWxl | Oryza sativa |
| 3.2.1.68 | 490000 | - |
native enzyme, gel filtration, TSKgel G4000SWxl | Oryza sativa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.68 | Oryza sativa | - |
L. cv. Fujikari | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.68 | - |
Oryza sativa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.68 | 51.2 | - |
rabbit liver glycogen | Oryza sativa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.68 | amylopectin + H2O | - |
Oryza sativa | maltose + maltooligosaccharides | - |
r | |
| 3.2.1.68 | glycogen + H2O | - |
Oryza sativa | maltose + maltooligosaccharides | - |
r | |
| 3.2.1.68 | phythoglycogen + H2O | - |
Oryza sativa | ? | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.68 | oligomer | probably homo-tetramer and homohexamer | Oryza sativa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.68 | rice isoamylase | - |
Oryza sativa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.68 | 30 | - |
- |
Oryza sativa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.68 | 10 | 40 | temperature treatment does not influence the enzyme activity | Oryza sativa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.68 | 6.5 | 7 | - |
Oryza sativa |
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