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Literature summary extracted from

  • Lee, E.Y.C.; Whelan, W.J.
    Glycogen and starch debranching enzymes (1972), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 5, 191-234.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.33 analysis useful in structural determination of glycogen and amylopectin Oryctolagus cuniculus
3.2.1.33 medicine diagnosis, even prenatal, of type III glycogen storage disease: marked decrease or absence of amylo-1,6-glucosidase, glycogen debranching enzyme Homo sapiens
3.2.1.68 analysis
-
 Pseudomonas amyloderamosa
3.2.1.68 analysis structural determination of glycogen and starch components Cytophaga sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.33 additional information
-
-
 Saccharomyces cerevisiae
3.2.1.33 additional information
-
-
 Oryctolagus cuniculus
3.2.1.33 70000
-
 glycogen debranching enzyme, three basic subunits, 1 * 122000, 1 * 85000, 1 * 70000, carboxymethylation prior to SDS-PAGE and gel electrophoresis in the presence of urea Saccharomyces cerevisiae
3.2.1.33 85000
-
 glycogen debranching enzyme, three basic subunits, 1 * 122000, 1 * 85000, 1 * 70000, carboxymethylation prior to SDS-PAGE and gel electrophoresis in the presence of urea Saccharomyces cerevisiae
3.2.1.33 122000
-
 glycogen debranching enzyme, three basic subunits, 1 * 122000, 1 * 85000, 1 * 70000, carboxymethylation prior to SDS-PAGE and gel electrophoresis in the presence of urea Saccharomyces cerevisiae
3.2.1.33 210000
-
 glycogen debranching enzyme, gel filtration and PAGE Saccharomyces cerevisiae
3.2.1.33 267000 279000 glycogen debranching enzyme, equilibrium ultracentrifugation Oryctolagus cuniculus
3.2.1.33 280000
-
-
 Saccharomyces cerevisiae
3.2.1.33 280000
-
 glycogen debranching enzyme, sedimentation equilibrium Saccharomyces cerevisiae
3.2.1.68 94000
-
-
 Pseudomonas amyloderamosa
3.2.1.68 120000
-
-
 Cytophaga sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O Mammalia phi-dextrin ?
-
 ?
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O Saccharomyces cerevisiae phi-dextrin ?
-
 ?
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O Oryctolagus cuniculus phi-dextrin ?
-
 ?
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O Oryctolagus cuniculus total degradation of glycogen is accomplished by the combined action of glycogen phosphorylase and glycogen debranching enzyme ?
-
 ?
3.2.1.33 additional information Mammalia glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen ?
-
 ?
3.2.1.33 additional information Saccharomyces cerevisiae glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen ?
-
 ?
3.2.1.33 additional information Oryctolagus cuniculus glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen ?
-
 ?
3.2.1.33 additional information Mammalia glycogen debranching system, indirect debranching enzymes, is the dominant type of glycogen debranching mechanism, where glycogen is stored ?
-
 ?
3.2.1.68 starch + H2O Pseudomonas amyloderamosa
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.33 Homo sapiens
-
-
-
3.2.1.33 Mammalia
-
 generally present in mammalian tissues
-
3.2.1.33 Oryctolagus cuniculus
-
-
-
3.2.1.33 Saccharomyces cerevisiae
-
-
-
3.2.1.33 Saccharomyces cerevisiae
-
 baker's yeast
-
3.2.1.68 Cytophaga sp.
-
-
-
3.2.1.68 Pseudomonas amyloderamosa
-
 gram-negative soil bacterium, strain SB-15
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.33 glycogen debranching enzyme Saccharomyces cerevisiae
3.2.1.33 glycogen debranching enzyme Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.33 erythrocyte
-
 Mammalia
-
3.2.1.33 leukocyte
-
 Mammalia
-
3.2.1.33 leukocyte
-
 Homo sapiens
-
3.2.1.33 liver
-
 Mammalia
-
3.2.1.33 liver
-
 Homo sapiens
-
3.2.1.33 liver
-
 Oryctolagus cuniculus
-
3.2.1.33 muscle
-
 Mammalia
-
3.2.1.33 muscle
-
 Homo sapiens
-
3.2.1.33 muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.33 4
-
 substrate: glycogen phosphorylase-limit dextrin Oryctolagus cuniculus
3.2.1.33 6
-
-
 Saccharomyces cerevisiae
3.2.1.33 7.6
-
-
 Oryctolagus cuniculus
3.2.1.33 8.3
-
-
 Oryctolagus cuniculus
3.2.1.33 8.4
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.33 2 D-glucose
-
 Oryctolagus cuniculus 6-O-alpha-D-glucosyl-D-glucose + H2O isomaltose r
3.2.1.33 63-alpha-glucosyl maltotetraose + H2O branched pentasaccharide "fast B5" Mammalia maltotetraose + D-glucose
-
 r
3.2.1.33 63-alpha-glucosyl maltotetraose + H2O branched pentasaccharide "fast B5" Oryctolagus cuniculus maltotetraose + D-glucose
-
 r
3.2.1.33 63-alpha-maltotriosyl maltotetraose + H2O branched heptasaccharide B7 Mammalia ?
-
 ?
3.2.1.33 63-alpha-maltotriosyl maltotetraose + H2O branched heptasaccharide B7 Oryctolagus cuniculus ?
-
 ?
3.2.1.33 alpha-(1-6)-glucosyl cyclohexaamylose + H2O 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin Saccharomyces cerevisiae cyclohexaamylose + D-glucose
-
 r
3.2.1.33 alpha-(1-6)-glucosyl cyclohexaamylose + H2O 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin Mammalia cyclohexaamylose + D-glucose alpha-Schardinger dextrin r
3.2.1.33 alpha-(1-6)-glucosyl cyclohexaamylose + H2O 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin Saccharomyces cerevisiae cyclohexaamylose + D-glucose alpha-Schardinger dextrin r
3.2.1.33 alpha-(1-6)-glucosyl cyclohexaamylose + H2O 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin Oryctolagus cuniculus cyclohexaamylose + D-glucose alpha-Schardinger dextrin r
3.2.1.33 alpha-dextrin + H2O
-
 Mammalia alpha-dextrin + D-glucose
-
 ?
3.2.1.33 alpha-dextrin + H2O
-
 Saccharomyces cerevisiae alpha-dextrin + D-glucose
-
 ?
3.2.1.33 amylopectin + H2O very poor substrate Oryctolagus cuniculus amylopectin + D-glucose partially debranched ?
3.2.1.33 amylopectin + H2O slowly hydrolyzed Mammalia amylopectin + D-glucose
-
 ?
3.2.1.33 amylopectin + H2O slowly hydrolyzed Saccharomyces cerevisiae amylopectin + D-glucose
-
 ?
3.2.1.33 amylopectin + H2O slowly hydrolyzed Saccharomyces cerevisiae amylopectin + D-glucose partially debranched ?
3.2.1.33 amylopectin + H2O phi-dextrin, beta-dextrin and phi-beta-dextrin from amylopectin Saccharomyces cerevisiae amylopectin + D-glucose partially debranched ?
3.2.1.33 amylopectin + H2O phi-dextrin, beta-dextrin and phi-beta-dextrin from amylopectin Oryctolagus cuniculus amylopectin + D-glucose partially debranched ?
3.2.1.33 amylose + H2O
-
 Saccharomyces cerevisiae ?
-
 r
3.2.1.33 amylose + H2O
-
 Oryctolagus cuniculus ?
-
 r
3.2.1.33 beta-amylase limit dextrin + H2O beta-dextrin Saccharomyces cerevisiae limit dextrin + D-glucose
-
 r
3.2.1.33 beta-amylase limit dextrin + H2O beta-dextrin Oryctolagus cuniculus limit dextrin + D-glucose
-
 r
3.2.1.33 D-glucose + maltooligosaccharide
-
 Saccharomyces cerevisiae maltooligosaccharide branched r
3.2.1.33 D-glucose + maltooligosaccharide
-
 Oryctolagus cuniculus maltooligosaccharide branched r
3.2.1.33 glycogen + H2O rabbit liver glycogen: very poor substrate, shellfish glycogen: not a substrate Oryctolagus cuniculus glycogen + D-glucose
-
 r
3.2.1.33 glycogen + H2O native glycogen from shellfish and rabbit liver Saccharomyces cerevisiae glycogen + D-glucose
-
 r
3.2.1.33 glycogen + H2O reverse reaction: incorporation of glucose into glycogen Mammalia glycogen + D-glucose
-
 r
3.2.1.33 glycogen + H2O reverse reaction: incorporation of glucose into glycogen Homo sapiens glycogen + D-glucose
-
 r
3.2.1.33 glycogen + H2O reverse reaction: incorporation of glucose into glycogen Saccharomyces cerevisiae glycogen + D-glucose
-
 r
3.2.1.33 glycogen + H2O reverse reaction: incorporation of glucose into glycogen Oryctolagus cuniculus glycogen + D-glucose
-
 r
3.2.1.33 glycogen + H2O rabbit liver glycogen: slowly Oryctolagus cuniculus glycogen + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O best substrate Mammalia limit dextrin + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O best substrate Saccharomyces cerevisiae limit dextrin + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O best substrate Oryctolagus cuniculus limit dextrin + D-glucose partially debranched glycogen r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O phi-dextrin Mammalia limit dextrin + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O phi-dextrin Saccharomyces cerevisiae limit dextrin + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O phi-dextrin Oryctolagus cuniculus limit dextrin + D-glucose partially debranched glycogen r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O phi-dextrin Mammalia ?
-
 ?
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O phi-dextrin Saccharomyces cerevisiae ?
-
 ?
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O phi-dextrin Oryctolagus cuniculus ?
-
 ?
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O total degradation of glycogen is accomplished by the combined action of glycogen phosphorylase and glycogen debranching enzyme Oryctolagus cuniculus ?
-
 ?
3.2.1.33 additional information substrate specificities Mammalia ?
-
 ?
3.2.1.33 additional information substrate specificities Saccharomyces cerevisiae ?
-
 ?
3.2.1.33 additional information substrate specificities Oryctolagus cuniculus ?
-
 ?
3.2.1.33 additional information 63-alpha-glucosyl maltotriose: not a substrate Oryctolagus cuniculus ?
-
 ?
3.2.1.33 additional information panose: not a substrate Saccharomyces cerevisiae ?
-
 ?
3.2.1.33 additional information pullulan: not a substrate Mammalia ?
-
 ?
3.2.1.33 additional information glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen Mammalia ?
-
 ?
3.2.1.33 additional information glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen Saccharomyces cerevisiae ?
-
 ?
3.2.1.33 additional information glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen Oryctolagus cuniculus ?
-
 ?
3.2.1.33 additional information glycogen debranching system, indirect debranching enzymes, is the dominant type of glycogen debranching mechanism, where glycogen is stored Mammalia ?
-
 ?
3.2.1.33 phosphorylase beta-amylase limit dextrin + H2O limit dextrin, beta-phi-dextrin, derived from the action of beta-amylase on the phosphorylase limit dextrin Saccharomyces cerevisiae limit dextrin + D-glucose
-
 r
3.2.1.33 phosphorylase beta-amylase limit dextrin + H2O limit dextrin, beta-phi-dextrin, derived from the action of beta-amylase on the phosphorylase limit dextrin Oryctolagus cuniculus limit dextrin + D-glucose
-
 r
3.2.1.68 amylopectin + H2O waxy maize amylopectin, potato amylopectin, amylopectin beta-dextrin Pseudomonas amyloderamosa maltose + maltooligosaccharides
-
 ?
3.2.1.68 amylopectin + H2O or the beta-limit dextrins, cleaves the branching points completely in vitro Pseudomonas amyloderamosa maltose + maltooligosaccharides
-
 ?
3.2.1.68 amylopectin phi-dextrin + H2O substrate on which all 3 types of debranching enzymes act Pseudomonas amyloderamosa maltotetraose
-
 ?
3.2.1.68 glycogen + H2O cleaves the branching points completely in vitro Pseudomonas amyloderamosa maltose + maltooligosaccharides
-
 ?
3.2.1.68 glycogen + H2O oyster glycogen, glycogen beta-dextrin, rabbit liver glycogen Pseudomonas amyloderamosa maltose + maltooligosaccharides
-
 ?
3.2.1.68 glycogen + H2O or the beta-limit dextrins Pseudomonas amyloderamosa maltose + maltooligosaccharides
-
 ?
3.2.1.68 additional information direct debranching enzyme, attacks unmodified glycogen and/or amylopectin with hydrolysis of the 1,6-bond, smallest substrate is not known Pseudomonas amyloderamosa ?
-
 ?
3.2.1.68 starch + H2O
-
 Pseudomonas amyloderamosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.33 More evidence for a subunit of 120000 Oryctolagus cuniculus
3.2.1.33 trimer
-
 Saccharomyces cerevisiae
3.2.1.33 trimer glycogen debranching enzyme, three basic subunits, 1 * 122000, 1 * 85000, 1 * 70000, carboxymethylation prior to SDS-PAGE and gel electrophoresis in the presence of urea Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.33 glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Mammalia
3.2.1.33 glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Homo sapiens
3.2.1.33 glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Saccharomyces cerevisiae
3.2.1.33 glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Oryctolagus cuniculus
3.2.1.68 Cythophaga isoamylase
-
 Cytophaga sp.
3.2.1.68 plant isoamylase
-
-
3.2.1.68 yeast isoamylase
-
-

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.33 additional information
-
 pH-optimum depends on type of buffer Oryctolagus cuniculus
3.2.1.33 additional information
-
 pH-optimum depends on substrate and type of buffer, overview Oryctolagus cuniculus
3.2.1.33 6 6.6
-
 Saccharomyces cerevisiae
3.2.1.33 6.6
-
 anionic buffer, substrate: glycogen phosphorylase limit dextrin Oryctolagus cuniculus
3.2.1.33 7.2
-
 cationic buffer, substrate: glycogen phosphorylase limit dextrin Oryctolagus cuniculus