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Literature summary extracted from

  • Igarashi, T.; Takahashi, M.; Yamamoto, A.; Etoh, Y.; Takamori, K.
    Purification and characterization of levanase from Actinomyces viscosus ATCC19246 (1987), Infect. Immun., 55, 3001-3005.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.65 medicine pathogenicity of periodontal disease Actinomyces viscosus
3.2.1.65 medicine mitogenic for human B lymphocytes and activates the alternative complement pathway Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.65 Ba2+
-
 Actinomyces viscosus
3.2.1.65 EDTA
-
 Actinomyces viscosus
3.2.1.65 Fe3+
-
 Actinomyces viscosus
3.2.1.65 Hg2+
-
 Bacillus subtilis
3.2.1.65 Mn2+
-
 Actinomyces viscosus
3.2.1.65 Zn2+
-
 Actinomyces viscosus
3.2.1.154 BaCl2 1 mM, 64% inhibition Actinomyces viscosus
3.2.1.154 EDTA 1 mM, complete inhibition Actinomyces viscosus
3.2.1.154 FeCl3 1 mM, 70% inhibition Actinomyces viscosus
3.2.1.154 HgCl2 1 mM, complete inhibition Actinomyces viscosus
3.2.1.154 MnCl2 1 mM, 19% inhibition Actinomyces viscosus
3.2.1.154 ZnCl2 1 mM, 93% inhibition Actinomyces viscosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.65 extracellular
-
 Bacillus subtilis
-
-
3.2.1.65 extracellular
-
 Actinomyces viscosus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.65 89000
-
 SDS-PAGE Actinomyces viscosus
3.2.1.154 89000
-
 x * 89000, SDS-PAGE Actinomyces viscosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.65 levans + H2O Bacillus subtilis
-
 oligofructosides
-
 ?
3.2.1.65 levans + H2O Actinomyces viscosus
-
 oligofructosides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.65 Actinomyces viscosus
-
-
-
3.2.1.65 Bacillus subtilis
-
-
-
3.2.1.154 Actinomyces viscosus
-
 ATCC 19246
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.65
-
 Actinomyces viscosus
3.2.1.154
-
 Actinomyces viscosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.154 culture supernatant
-
 Actinomyces viscosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.65 851
-
-
 Actinomyces viscosus
3.2.1.154 851.2
-
-
 Actinomyces viscosus

Storage Stability

EC Number Storage Stability Organism
3.2.1.65 4°C, phosphate buffer, pH 6.0 Actinomyces viscosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.65 inulin + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.65 inulin + H2O
-
 Actinomyces viscosus ?
-
 ?
3.2.1.65 levan + H2O
-
 Bacillus subtilis oligofructosides
-
 ?
3.2.1.65 levan + H2O
-
 Actinomyces viscosus oligofructosides
-
 ?
3.2.1.65 levans + H2O
-
 Bacillus subtilis oligofructosides
-
 ?
3.2.1.65 levans + H2O
-
 Actinomyces viscosus oligofructosides
-
 ?
3.2.1.65 sucrose + H2O
-
 Bacillus subtilis D-fructose + D-glucose
-
 ?
3.2.1.65 sucrose + H2O
-
 Actinomyces viscosus D-fructose + D-glucose
-
 ?
3.2.1.154 levan + H2O
-
 Actinomyces viscosus ?
-
 ?
3.2.1.154 additional information no cleavage of inulin, dextran, sucrsoe, raffinose and melezitose Actinomyces viscosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.65 monomer SDS-PAGE Actinomyces viscosus
3.2.1.154 ? x * 89000, SDS-PAGE Actinomyces viscosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.154 levanase
-
 Actinomyces viscosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.65 45
-
 not active above 55°C Actinomyces viscosus
3.2.1.154 45
-
-
 Actinomyces viscosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.154 35 50 35°C: about 50% of maximal activity, 50°C: about 80% of maximal activity Actinomyces viscosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.65 4
-
 not active below Actinomyces viscosus
3.2.1.65 6
-
-
 Actinomyces viscosus
3.2.1.154 6
-
-
 Actinomyces viscosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.154 5 7.5 pH 5.0: about 50% of maximal activity, pH 7.5: about 60% of maximal activity Actinomyces viscosus