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Literature summary extracted from

  • Aminoff, D.; Frurukawa, K.
    Enzymes that destroy blood group specificity. I. Purification and properties of alpha-L-fucosidase from Clostridium perfringens (1970), J. Biol. Chem., 245, 1659-1669.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.63 dialysis, water: rapid loss of activity, buffers: precipitation with considerable inactivation below pH 5.5 Clostridium perfringens
3.2.1.63 lyophilization causes inactivation Clostridium perfringens
3.2.1.63 repeated freezing and thawing causes inactivation Clostridium perfringens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.63 Cu2+
-
 Clostridium perfringens
3.2.1.63 EDTA
-
 Clostridium perfringens
3.2.1.63 Fe2+
-
 Clostridium perfringens
3.2.1.63 Fe3+
-
 Clostridium perfringens
3.2.1.63 Hg2+
-
 Clostridium perfringens
3.2.1.63 iodoacetamide
-
 Clostridium perfringens
3.2.1.63 p-chloromercuribenzoate
-
 Clostridium perfringens
3.2.1.63 Zn2+
-
 Clostridium perfringens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.63 200000
-
 value greater than, multiple isozymic forms Clostridium perfringens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.63 submaxillary glycoprotein + H2O Clostridium perfringens degradation of blood group substances L-fucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.63 Clostridium perfringens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.63
-
 Clostridium perfringens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.63 70
-
-
 Clostridium perfringens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.63 additional information not: p-nitrophenyl-alpha-L-fucoside, 2-O-, 3-O- and 4-O-alpha-L-fucopyranosylfucoses, 3-O- and 4-O-alpha-L-fucopyranosyl-D-galactoses, action on oligosaccharides and glycoproteins, not: simple methyl nitrophenyl fucosides Clostridium perfringens ?
-
 ?
3.2.1.63 O-alpha-L-fucose-(1-2)-O-beta-D-galactose-(1-4)-D-glucose + H2O i.e. 2-O-alpha-L-fucosyllactose Clostridium perfringens L-fucose + lactose
-
 ?
3.2.1.63 submaxillary glycoprotein + H2O degradation of blood group substances Clostridium perfringens L-fucose + ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.63 6
-
-
 Clostridium perfringens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.63 4.5 8
-
 Clostridium perfringens