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Literature summary extracted from

  • Tung, K.K.; Rosenthal, A.; Nordin, J.H.
    Enzymes that hydrolyze fungal cell wall polysaccharides. II. Purification and properties of mycodextranase, an endo -D-(1-4) glucanase from Penicillium melinii (1971), J. Biol. Chem., 246, 6722-6736.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.61 Ag+ fast enzyme Penicillium melinii
3.2.1.61 Hg+ fast enzyme Penicillium melinii
3.2.1.61 maltose
-
 Penicillium melinii
3.2.1.61 maltotriose
-
 Penicillium melinii
3.2.1.61 N-bromosuccinimide fast enzyme Penicillium melinii
3.2.1.61 N-Chlorosuccinimide fast enzyme Penicillium melinii
3.2.1.61 nigeran tetrasaccharide
-
 Penicillium melinii
3.2.1.61 nigeran trisaccharide
-
 Penicillium melinii
3.2.1.61 nigerose
-
 Penicillium melinii
3.2.1.61 Urea
-
 Penicillium melinii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.61 40000
-
 sedimentation equilibrium fast and slow enzyme Penicillium melinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.61 nigeran + H2O Penicillium melinii
-
 nigerose + 4-alpha-D-nigerosylglucose
-
 ?
3.2.1.61 nigeran + H2O Penicillium melinii QM 1931
-
 nigerose + 4-alpha-D-nigerosylglucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.61 Penicillium melinii
-
 QM 1931
-
3.2.1.61 Penicillium melinii QM 1931
-
 QM 1931
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.61 glycoprotein fast form: 16% carbohydrate, slow form: 15% carbohydrate, only mannose and glucose Penicillium melinii

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.61 fast and slow enzyme, different electrophoretic mobilities Penicillium melinii

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.61 nigerohexaose + H2O = nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose endo-multichain mechanism Penicillium melinii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.61 27
-
-
 Penicillium melinii

Storage Stability

EC Number Storage Stability Organism
3.2.1.61 4°C, 2 months, no loss of activity, fast and slow enzyme Penicillium melinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.61 nigeran + H2O both, fast and slow enzyme Penicillium melinii nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose no production of glucose ?
3.2.1.61 nigeran + H2O both, fast and slow enzyme Penicillium melinii QM 1931 nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose no production of glucose ?
3.2.1.61 nigeran + H2O
-
 Penicillium melinii nigerose + 4-alpha-D-nigerosylglucose
-
 ?
3.2.1.61 nigeran + H2O
-
 Penicillium melinii QM 1931 nigerose + 4-alpha-D-nigerosylglucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.61 monomer 1 * 40000, guanidine hydrochloride treatment Penicillium melinii
3.2.1.61 More fast and slow enzyme have similar but not identical amino acid compositions Penicillium melinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.61 70
-
 fast and slow enzyme Penicillium melinii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.61 23 80 23°C: fast enzyme 7%, slow enzyme 16% of maximal activity, 80°C: fast enzyme 50%, slow enzyme 30% of maximal activity Penicillium melinii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.61 2 9 fast and slow enzyme pH 2 15%, pH 9 75% of maximal activity Penicillium melinii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.61 4 5 stable between Penicillium melinii