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Literature summary extracted from

  • Sakano, Y.; Kashiyama, E.; Kobayashi, T.
    Purification of alpha-maltotetraose-forming exo-amylase of Pseudomonas stutzeri-two forms of the amylase and their enzymatic properties (1983), Agric. Biol. Chem., 47, 1761-1768.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.60 Ca2+ stabilizes Pseudomonas stutzeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.60 Al3+ F1 and F2 form 30% inhibition Pseudomonas stutzeri
3.2.1.60 Co2+ F1 and F2 form 47% inhibition Pseudomonas stutzeri
3.2.1.60 Cu2+ F1 and F2 form 92% inhibition Pseudomonas stutzeri
3.2.1.60 Fe2+ F1 form, 96% F2 form, 98% inhibition Pseudomonas stutzeri
3.2.1.60 Fe3+ F1 form, 75% F2 form, 76% inhibition Pseudomonas stutzeri
3.2.1.60 Hg2+ F1 and F2 form, complete inhibition Pseudomonas stutzeri
3.2.1.60 N-bromosuccinimide F1 form, pH 6.5 complete inhibition, pH 8.0, 93% inhibition, F2 form, pH 6.5, 95% inhibition, pH 8.0, 28% inhibition Pseudomonas stutzeri
3.2.1.60 Ni2+ F1 form 62%, F2 form 67% inhibition Pseudomonas stutzeri
3.2.1.60 Zn2+ F1 form 94%, F2 form 96% inhibition Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.60 0.32
-
 amylose F1 and F2 form Pseudomonas stutzeri
3.2.1.60 5.7
-
 starch F2 form Pseudomonas stutzeri
3.2.1.60 5.9
-
 starch shellfish glycogen, F1 and F2 form Pseudomonas stutzeri
3.2.1.60 5.9
-
 starch F1 form Pseudomonas stutzeri
3.2.1.60 6.7
-
 oyster glycogen F2 form Pseudomonas stutzeri
3.2.1.60 7.1
-
 oyster glycogen F1form Pseudomonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.60 55000
-
 F1 and F2 form, SDS-PAGE Pseudomonas stutzeri

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.60 Pseudomonas stutzeri
-
 NRRL B-3389
-
3.2.1.60 Pseudomonas stutzeri NRRL B-3389
-
 NRRL B-3389
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.60 two active forms, F1 and F2 Pseudomonas stutzeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.60 102
-
 F2 form Pseudomonas stutzeri
3.2.1.60 104
-
 F1 form Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.60 amylopectin + H2O
-
 Pseudomonas stutzeri maltotetraose + ?
-
 ?
3.2.1.60 amylopectin + H2O
-
 Pseudomonas stutzeri NRRL B-3389 maltotetraose + ?
-
 ?
3.2.1.60 amylose + H2O
-
 Pseudomonas stutzeri maltotetraose
-
 ?
3.2.1.60 amylose + H2O
-
 Pseudomonas stutzeri NRRL B-3389 maltotetraose
-
 ?
3.2.1.60 glycogen + H2O oyster and shellfish glycogen Pseudomonas stutzeri maltotetraose
-
 ?
3.2.1.60 glycogen + H2O oyster and shellfish glycogen Pseudomonas stutzeri NRRL B-3389 maltotetraose
-
 ?
3.2.1.60 oyster glycogen + H2O
-
 Pseudomonas stutzeri maltotetraose + ?
-
 ?
3.2.1.60 oyster glycogen + H2O
-
 Pseudomonas stutzeri NRRL B-3389 maltotetraose + ?
-
 ?
3.2.1.60 starch + H2O
-
 Pseudomonas stutzeri maltotetraose
-
 ?
3.2.1.60 starch + H2O
-
 Pseudomonas stutzeri NRRL B-3389 maltotetraose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.60 45
-
 pH 8.0, F1 and F2 form Pseudomonas stutzeri

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.60 25 65 F1 and F2 form 25°C, 60% of maximal activity, 65°C, 20% of maximal activity Pseudomonas stutzeri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.60 40
-
 F1 and F2 form, stable below on the treatment at pH 8.0, 60 min in the presence or absence of 5 mM CaCl2 Pseudomonas stutzeri
3.2.1.60 55
-
 F1 and F2 form, complete loss of activity in the absence of Ca2+ Pseudomonas stutzeri
3.2.1.60 60
-
 F1 and F2 form, complete loss of activity in the presence of Ca2+ Pseudomonas stutzeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.60 7.8 8.2 F1 and F2 form Pseudomonas stutzeri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.60 5.5 11 30°C, 60 min, presence of CaCl2, stable between Pseudomonas stutzeri
3.2.1.60 6.5 8.5 absence of Ca2+, 30°C, 60 min stable between Pseudomonas stutzeri