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Literature summary extracted from

  • Nagata, S.; Sawatani, M.; Kuriyama, M.; Misono, H.; Nagasaki, S.
    Purification and characterization of nonlytic endo-beta-1,3-glucanase I from Flavobacterium dormitator var. glucanolyticae (1990), Agric. Biol. Chem., 54, 2107-2114.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.6 stable against proteolysis, full activity after papain treatment for 20 h Flavobacterium dormitator

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.6 Ag+ 87% of activity with 10 mM Flavobacterium dormitator
3.2.1.6 Hg2+ 99% inhibition with 10 mM Flavobacterium dormitator

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.6 Ni2+ 1.5fold activity in the presence of 10 mM NiSO4 Flavobacterium dormitator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.6 27000
-
 SDS-PAGE Flavobacterium dormitator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.6 laminarin + H2O Flavobacterium dormitator use as carbon source ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Flavobacterium dormitator
-
 var. glucanolyticae FA-5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.6
-
 Flavobacterium dormitator

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.6 25
-
 purified enzyme, laminarin as substrate Flavobacterium dormitator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 beta-glucan + H2O yeast glucan Flavobacterium dormitator laminaridextrins
-
 ?
3.2.1.6 curdlan + H2O
-
 Flavobacterium dormitator laminaripentaose
-
 ?
3.2.1.6 laminarin + H2O
-
 Flavobacterium dormitator D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose D-glucose + laminaribiose ?
3.2.1.6 laminarin + H2O
-
 Flavobacterium dormitator D-glucose + laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + laminarihexaose laminaritriose + D-glucose ?
3.2.1.6 laminarin + H2O use as carbon source Flavobacterium dormitator ?
-
 ?
3.2.1.6 lichenin + H2O weak activity of enzyme I Flavobacterium dormitator beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->4)]-beta-D-glucopyranose + lichenin oligomers
-
 ?
3.2.1.6 pachyman + H2O weak activity of enzyme I Flavobacterium dormitator laminarisaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.6 monomer 1 * 27000 Flavobacterium dormitator

Synonyms

EC Number Synonyms Comment Organism
3.2.1.6 nonlytic endo-beta-1,3-glucanase I
-
 Flavobacterium dormitator

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.6 40 55 55°C laminarin as substrate Flavobacterium dormitator

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.6 50 70 enzyme I: full activity on heating at 55°C at pH 6.0 for 10 min, substantial loss of activity by heating at 70°C for 10 min Flavobacterium dormitator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.6 5.5
-
-
 Flavobacterium dormitator

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.6 6 9
-
 Flavobacterium dormitator