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Literature summary extracted from

  • Nagasaki, S.; Saito, K.; Yamamoto, S.
    Purification and characterization of an exo beta-1,3-glucanase from a Fungi imperfecti (1977), Agric. Biol. Chem., 41, 493-502.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.58 CoSO4
-
 Fungi imperfecti
3.2.1.58 CuSO4
-
 Fungi imperfecti
3.2.1.58 D-glucono-delta-lactone
-
 Fungi imperfecti
3.2.1.58 ethanol inhibition reversible when up to 80% ethanol concentration used Fungi imperfecti
3.2.1.58 Hg2+
-
 Fungi imperfecti
3.2.1.58 HgCl2
-
 Fungi imperfecti
3.2.1.58 Li2SO4
-
 Fungi imperfecti
3.2.1.58 MnCl2
-
 Fungi imperfecti
3.2.1.58 N-bromosuccinimide
-
 Fungi imperfecti
3.2.1.58 Na2HAsO4
-
 Fungi imperfecti
3.2.1.58 p-chloromercuribenzoate
-
 Fungi imperfecti
3.2.1.58 ZnSO4
-
 Fungi imperfecti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.58 0.066
-
 Laminarin
-
 Fungi imperfecti
3.2.1.58 0.83
-
 Laminarihexaose
-
 Fungi imperfecti
3.2.1.58 2.3
-
 Laminaripentaose
-
 Fungi imperfecti
3.2.1.58 18.6
-
 laminaritetraose
-
 Fungi imperfecti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.58 AgNO3 activity enhanced by 15% Fungi imperfecti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.58 43000
-
 gel filtration Fungi imperfecti

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.58 Fungi imperfecti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.58
-
 Fungi imperfecti

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.58 169.4
-
-
 Fungi imperfecti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.58 glucan + H2O yeast glucan Fungi imperfecti D-glucose + gentiobiose
-
 ?
3.2.1.58 laminarihexaose + H2O
-
 Fungi imperfecti D-glucose + laminarioligosaccharides
-
 ?
3.2.1.58 laminarin + H2O
-
 Fungi imperfecti D-glucose + laminaribiose + gentiobiose + oligosaccharides + laminaritriose different combinations and amounts of following products: D-glucose (main product), laminaribiose, gentiobiose, oligosaccharides, laminaritriose ?
3.2.1.58 laminaripentaose + H2O
-
 Fungi imperfecti D-glucose + laminaribiose + laminaritriose + laminaritetraose
-
 ?
3.2.1.58 laminaritetraose + H2O
-
 Fungi imperfecti D-glucose + laminaribiose + laminaritriose
-
 ?
3.2.1.58 pachyman + H2O
-
 Fungi imperfecti D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.58 50
-
-
 Fungi imperfecti

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.58 35
-
 stable below 35°C Fungi imperfecti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.58 6
-
-
 Fungi imperfecti