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Literature summary extracted from

  • Yoshida, A.; Iwasaki, Y.; Akiba, T.; Horikoshi, K.
    Purification and properties of cyclomaltodextrinase from alkalophilic Bacillus sp. (1991), J. Ferment. Bioeng., 71, 226-229.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.54 Ag+
-
 Niallia circulans
3.2.1.54 Cu2+
-
 Niallia circulans
3.2.1.54 dodecylbenzenesulfonate 0.1% Niallia circulans
3.2.1.54 Hg2+
-
 Niallia circulans
3.2.1.54 p-chloromercuribenzoate
-
 Niallia circulans
3.2.1.54 SDS 0.1% Niallia circulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.54 0.8
-
 maltoheptaose
-
 Niallia circulans
3.2.1.54 0.8
-
 gamma-cyclodextrin
-
 Niallia circulans
3.2.1.54 1.2
-
 maltopentaose
-
 Niallia circulans
3.2.1.54 1.3
-
 maltohexaose
-
 Niallia circulans
3.2.1.54 1.8
-
 beta-cyclodextrin
-
 Niallia circulans
3.2.1.54 3.2
-
 alpha-cyclodextrin
-
 Niallia circulans
3.2.1.54 7.4
-
 maltotetraose
-
 Niallia circulans
3.2.1.54 12.5
-
 maltotriose
-
 Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 67000
-
 2 * 67000, molecular weight estimated to be 126000 by gel filtration, 67000 by SDS-PAGE Niallia circulans
3.2.1.54 126000
-
 MW estimation, gel filtration Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Niallia circulans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 purified 252fold Niallia circulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.54 14.8
-
 gel filtration Niallia circulans

Storage Stability

EC Number Storage Stability Organism
3.2.1.54 4°C, one month without any loss of activity Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 alpha-cyclodextrin + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.54 beta-cyclodextrin + H2O
-
 Niallia circulans maltose + maltotriose + maltotetraose + maltopentaose + maltoheptaose + glucose
-
 ?
3.2.1.54 cyclomaltodextrin + H2O
-
 Niallia circulans maltodextrin maltooligosaccharides ?
3.2.1.54 gamma-cyclodextrin + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.54 maltodextrins + H2O
-
 Niallia circulans maltose + glucose
-
 ?
3.2.1.54 maltoheptaose + H2O
-
 Niallia circulans D-glucose + maltose + ?
-
 ?
3.2.1.54 maltohexaose + H2O
-
 Niallia circulans maltotetraose + maltose
-
 ?
3.2.1.54 maltopentaose + H2O
-
 Niallia circulans maltose + maltotriose
-
 ?
3.2.1.54 maltotetraose + H2O
-
 Niallia circulans 2 maltose
-
 ?
3.2.1.54 maltotriose + H2O
-
 Niallia circulans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.54 dimer 2 * 67000, molecular weight estimated to be 126000 by gel filtration, 67000 by SDS-PAGE Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 50
-
-
 Niallia circulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.54 30 55 no activity at 60°C Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 6
-
-
 Niallia circulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.54 5 8
-
 Niallia circulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.54 6 10 40°C, 30 min stable Niallia circulans