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Literature summary extracted from

  • Frohwein, Y.Z.; Gatt, S.
    Isolation of beta-N-acetylhexosaminidase, beta-N-acetylglucosaminidase, and beta-N-acetylgalactosaminidase from calf brain (1967), Biochemistry, 6, 2775-2782.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 N-ethylmaleimide N-acetylgalactosaminidase Rattus norvegicus
3.2.1.52 N-ethylmaleimide N-acetylgalactosaminidase Bos taurus
3.2.1.53 N-ethylmaleimide 1mM solution increase the reaction rates 2-3fold Bos taurus

General Stability

EC Number General Stability Organism
3.2.1.53 cysteine, stabilization Bos taurus
3.2.1.53 dithiothreitol, stabilization Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 acetate inhibits N-acetylhexosaminidase Bos taurus
3.2.1.52 acetate inhibits N-acetylhexosaminidase Rattus norvegicus
3.2.1.52 Ag+ inhibits N-acetylhexosaminidase, -galactosaminidase and -glucosaminidase Bos taurus
3.2.1.52 Ag+ inhibits N-acetylhexosaminidase, -galactosaminidase and -glucosaminidase Rattus norvegicus
3.2.1.52 galactosamine inhibits N-acetylhexosaminidase Bos taurus
3.2.1.52 galactosamine inhibits N-acetylhexosaminidase Rattus norvegicus
3.2.1.52 glucosamine inhibits N-acetylhexosaminidase Bos taurus
3.2.1.52 glucosamine inhibits N-acetylhexosaminidase Rattus norvegicus
3.2.1.52 N-acetyl-D-galactosamine inhibits N-acetylhexosaminidase and -galactosaminidase Bos taurus
3.2.1.52 N-acetyl-D-galactosamine inhibits N-acetylhexosaminidase and -galactosaminidase Rattus norvegicus
3.2.1.52 N-acetyl-D-glucosamine inhibits N-acetylhexosaminidase and -glucosaminidase Bos taurus
3.2.1.52 N-acetyl-D-glucosamine inhibits N-acetylhexosaminidase and -glucosaminidase Rattus norvegicus
3.2.1.52 N-ethylmaleimide N-acetylglucosaminidase Bos taurus
3.2.1.52 N-ethylmaleimide N-acetylglucosaminidase Rattus norvegicus
3.2.1.52 p-hydroxymercuribenzoate inhibits N-acetylhexosaminidase, -galactosaminidase and -glucosaminidase Bos taurus
3.2.1.52 p-hydroxymercuribenzoate inhibits N-acetylhexosaminidase, -galactosaminidase and -glucosaminidase Rattus norvegicus
3.2.1.53 AgNO3
-
 Bos taurus
3.2.1.53 N-acetylgalactosamine competitive inhibition Bos taurus
3.2.1.53 N-acetylglucosamine no inhibition at N-acetylglucosamine concentration of 40 mM Bos taurus
3.2.1.53 p-hydroxymercuribenzoate
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.35
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside N-acetylgalactosaminidase Rattus norvegicus
3.2.1.52 0.35
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside N-acetylgalactosaminidase Bos taurus
3.2.1.52 0.54
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside N-acetylhexosaminidase Rattus norvegicus
3.2.1.52 0.54
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside N-acetylhexosaminidase Bos taurus
3.2.1.52 0.72
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside N-acetylglucosaminidase Rattus norvegicus
3.2.1.52 0.72
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside N-acetylglucosaminidase Bos taurus
3.2.1.52 0.8
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside N-acetylhexosaminidase Rattus norvegicus
3.2.1.52 0.8
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside N-acetylhexosaminidase Bos taurus
3.2.1.53 0.35
-
 4-nitrophenyl beta-acetylgalactosaminide
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Rattus norvegicus
-
 N-acetyl-D-hexosamines
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Bos taurus
-
 N-acetyl-D-hexosamines
-
 ?
3.2.1.53 N-acetyl-beta-D-galactosaminides + H2O Bos taurus
-
 ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.53 Acetone inactivation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Bos taurus
-
 calf
-
3.2.1.52 Rattus norvegicus
-
-
-
3.2.1.53 Bos taurus
-
 calf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 N-acetylglucosaminidase and -galactosaminidase Rattus norvegicus
3.2.1.52 N-acetylhexosaminidase: 63fold Rattus norvegicus
3.2.1.52 N-acetylhexosaminidase: 63fold, N-acetylglucosaminidase and -galactosaminidase Bos taurus
3.2.1.53
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 brain
-
 Rattus norvegicus
-
3.2.1.52 brain
-
 Bos taurus
-
3.2.1.53 brain
-
 Bos taurus
-
3.2.1.53 liver
-
 Bos taurus
-
3.2.1.53 spleen
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 0.03
-
 N-acetylhexosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Rattus norvegicus
3.2.1.52 0.03
-
 N-acetylhexosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Bos taurus
3.2.1.52 0.22
-
 N-acetylhexosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Rattus norvegicus
3.2.1.52 0.22
-
 N-acetylhexosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 -20°C, N-acetyl-glucosaminidase and -galactosaminidase completely lose activity after several days Rattus norvegicus
3.2.1.52 -20°C, N-acetyl-glucosaminidase and -galactosaminidase completely lose activity after several days Bos taurus
3.2.1.52 -20°C, N-acetyl-hexosaminidase, in presence of ammonium sulfate, after several weeks: precipitate devoid of enzymatic activity forms but can be removed by centrifugation, further purification: loss of activity after several days at -20°C Rattus norvegicus
3.2.1.52 -20°C, N-acetyl-hexosaminidase, in presence of ammonium sulfate, after several weeks: precipitate devoid of enzymatic activity forms but can be removed by centrifugation, further purification: loss of activity after several days at -20°C Bos taurus
3.2.1.53 -20 ºC, inactivation after several weeks Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 chitobiose + H2O
-
 Rattus norvegicus 2 D-glucosamine
-
 ?
3.2.1.52 chitobiose + H2O
-
 Bos taurus 2 D-glucosamine
-
 ?
3.2.1.52 lacto-N-triose + H2O
-
 Rattus norvegicus ?
-
 ?
3.2.1.52 lacto-N-triose + H2O
-
 Bos taurus ?
-
 ?
3.2.1.52 N-acetyl-beta-D-glucosaminide-1,4-muramic acid + H2O
-
 Rattus norvegicus ?
-
 ?
3.2.1.52 N-acetyl-beta-D-glucosaminide-1,4-muramic acid + H2O
-
 Bos taurus ?
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Rattus norvegicus N-acetyl-D-hexosamines
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Bos taurus N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Rattus norvegicus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Bos taurus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Rattus norvegicus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Bos taurus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 phenyl-N-acetyl-beta-D-glucosaminide + H2O
-
 Rattus norvegicus phenol + N-acetyl-beta-D-glucosaminide
-
 ?
3.2.1.52 phenyl-N-acetyl-beta-D-glucosaminide + H2O
-
 Bos taurus phenol + N-acetyl-beta-D-glucosaminide
-
 ?
3.2.1.52 trihexosylceramide + H2O
-
 Rattus norvegicus ?
-
 ?
3.2.1.52 trihexosylceramide + H2O
-
 Bos taurus ?
-
 ?
3.2.1.53 4-nitrophenyl beta-acetylgalactosaminide + H2O
-
 Bos taurus 4-nitrophenol + beta-acetylgalactosamine
-
 ?
3.2.1.53 beta-phenyl-N-acetylgalactosamines + H2O substrate specificity 85% Bos taurus ?
-
 ?
3.2.1.53 N-acetyl-beta-D-galactosaminides + H2O e.g. 4-methylumbelliferyl-beta-N-acetylgalactosaminide Bos taurus N-acetyl-D-galactosamine + an alcohol e.g. 4-methylumbelliferone ?
3.2.1.53 N-acetyl-beta-D-galactosaminides + H2O
-
 Bos taurus ?
-
 ?
3.2.1.53 p-nitrophenyl beta-N-acetylgalactosaminide + H2O
-
 Bos taurus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 3.8
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, N-acetylhexosaminidase Rattus norvegicus
3.2.1.52 3.8
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, N-acetylhexosaminidase Bos taurus
3.2.1.52 4.2
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, N-acetylhexosaminidase Rattus norvegicus
3.2.1.52 4.2
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, N-acetylhexosaminidase Bos taurus
3.2.1.52 5.2
-
 N-acetylglucosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Rattus norvegicus
3.2.1.52 5.2
-
 N-acetylglucosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Bos taurus
3.2.1.52 5.5
-
 N-acetylgalactosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Rattus norvegicus
3.2.1.52 5.5
-
 N-acetylgalactosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Bos taurus
3.2.1.53 5.5
-
 p-nitrophenyl beta-N-acetylgalactosaminide Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.53 4
-
 not active below, p-nitrophenyl beta-N-acetylgalactosaminide Bos taurus