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Literature summary extracted from

  • Schönert, S.; Buder, T.; Dahl, M.K.
    Identification and enzymatic characterization of the maltose-inducible alpha-glucosidase MalL (sucrase-isomaltase-maltase) of Bacillus subtilis (1998), J. Bacteriol., 180, 2574-2578.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.48 maltose
-
 Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.10 expression in Escherichia coli Bacillus subtilis
3.2.1.20 overproduced in Escherichia coli Bacillus subtilis
3.2.1.48 expression in Escherichia coli, the gene coding the enzyme has been identified in the ORF yvdL/malL Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.10 KCl 40% inhibition by 1 M Bacillus subtilis
3.2.1.10 NaCl 50% inhibition by 500 mM Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.10 0.13
-
 maltose
-
 Bacillus subtilis
3.2.1.10 0.21
-
 p-nitrophenyl-alpha-D-glucopyranoside
-
 Bacillus subtilis
3.2.1.10 0.45
-
 isomaltose
-
 Bacillus subtilis
3.2.1.10 10.2
-
 sucrose
-
 Bacillus subtilis
3.2.1.20 0.135
-
 maltose
-
 Bacillus subtilis
3.2.1.20 0.21
-
 p-nitrophenyl-alpha-D-glucoside
-
 Bacillus subtilis
3.2.1.20 0.455
-
 isomaltose
-
 Bacillus subtilis
3.2.1.20 10.2
-
 sucrose
-
 Bacillus subtilis
3.2.1.48 0.135
-
 maltose
-
 Bacillus subtilis
3.2.1.48 0.21
-
 p-nitrophenyl-alpha-D-glucoside
-
 Bacillus subtilis
3.2.1.48 0.455
-
 isomaltose
-
 Bacillus subtilis
3.2.1.48 10.2
-
 sucrose
-
 Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.10 oligosaccharide + H2O Bacillus subtilis direct function in maltose and isomaltose metabolism monosaccharide
-
 ?
3.2.1.20 additional information Bacillus subtilis maltose is required for induction. The enzyme may play a role in the breakdown of internal storage polysaccharides containing alpha-1,4 and alpha-1,6 bonds ?
-
 ?
3.2.1.48 maltose + H2O Bacillus subtilis
-
 2 glucose
-
 ?
3.2.1.48 sucrose + H2O Bacillus subtilis
-
 D-glucose + D-fructose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.10 Bacillus subtilis
-
-
-
3.2.1.20 Bacillus subtilis
-
-
-
3.2.1.48 Bacillus subtilis
-
 maltose-inducible alpha-glucosidase MalL, sucrase-isomaltase-maltase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.10
-
 Bacillus subtilis
3.2.1.20
-
 Bacillus subtilis
3.2.1.48 produced and purified in Escherichia coli RB791/pMalL Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.48 1.47
-
 maltose Bacillus subtilis
3.2.1.48 2.02
-
 isomaltose Bacillus subtilis
3.2.1.48 4.47
-
 sucrose Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
3.2.1.20 4°C, stable for at least 4 weeks Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.10 isomaltosaccharides + H2O n = 2, isomaltose Bacillus subtilis D-glucose
-
 ?
3.2.1.10 isomaltose + H2O
-
 Bacillus subtilis 2 D-glucose
-
 ?
3.2.1.10 maltose + H2O
-
 Bacillus subtilis 2 D-glucose
-
 ?
3.2.1.10 oligosaccharide + H2O direct function in maltose and isomaltose metabolism Bacillus subtilis monosaccharide
-
 ?
3.2.1.10 p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
 Bacillus subtilis D-glucose + p-nitrophenol
-
 ?
3.2.1.10 sucrose + H2O
-
 Bacillus subtilis D-glucose + D-fructose
-
 ?
3.2.1.20 isomaltose + H2O
-
 Bacillus subtilis 2 alpha-D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Bacillus subtilis alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 additional information the enzyme is a specific oligo-1,4-1,6-alpha-glucosidase Bacillus subtilis ?
-
 ?
3.2.1.20 additional information maltose is required for induction. The enzyme may play a role in the breakdown of internal storage polysaccharides containing alpha-1,4 and alpha-1,6 bonds Bacillus subtilis ?
-
 ?
3.2.1.20 p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
 Bacillus subtilis 4-nitrophenol + D-glucose
-
 ?
3.2.1.20 sucrose + H2O
-
 Bacillus subtilis D-fructose + alpha-D-glucose
-
 ?
3.2.1.48 isomaltose + H2O
-
 Bacillus subtilis 2 D-glucose
-
 ?
3.2.1.48 maltose + H2O
-
 Bacillus subtilis 2 glucose
-
 ?
3.2.1.48 maltose + H2O
-
 Bacillus subtilis 2 D-glucose
-
 ?
3.2.1.48 p-nitrophenyl-alpha-glucoside + H2O
-
 Bacillus subtilis p-nitrophenol + D-glucose
-
 ?
3.2.1.48 sucrose + H2O
-
 Bacillus subtilis D-glucose + D-fructose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.10 MalL
-
 Bacillus subtilis
3.2.1.10 oligo-1,4-1,6-alpha-glucosidase
-
 Bacillus subtilis
3.2.1.10 sucrase-isomaltase-maltase
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.10 7
-
 p-nitrophenyl-alpha-D-glucopyranoside as substrate Bacillus subtilis
3.2.1.48 7
-
 with PNPG as substrate, at pH 5.75 and 8.0 activity reduced to 50% Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.10 5.7 8 half-optima at pH 5.75 and 8.0 Bacillus subtilis