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Literature summary extracted from
- Inhibition of purified human sucrase and isomaltase by ethanolamine derivatives (1996), Biol. Pharm. Bull., 19, 341-344.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.48 | medicine | sucrase-isomaltase SI is a marker enzyme of the absorptive villose cells in adult small intestine, human intestinal sucrase-isomaltase help develop a specific inhibitor of sucrase, to retard the absorption of sucrose in the intestinal tract of diabetic patients | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.10 | 2-Amino-2-ethyl-1,3-propanediol | 80% inhibition of isomaltase activity by 50 mM | Homo sapiens |  |
| 3.2.1.10 | 2-amino-2-methyl-1,3-propanediol | almost complete inhibition of isomaltase activity by 50 mM | Homo sapiens | |
| 3.2.1.10 | diethanolamine | about 80% inhibition of isomaltase activity by 50 mM | Homo sapiens | |
| 3.2.1.10 | Monoethanolamine | about 70% inhibition of isomaltase activity by 50 mM | Homo sapiens | |
| 3.2.1.10 | triethanolamine | about 80% inhibition of isomaltase activity by 50 mM | Homo sapiens | |
| 3.2.1.10 | Tris | complete inhibition of isomaltase activity by 50 mM | Homo sapiens | |
| 3.2.1.48 | 2-Amino-2-ethyl-1,3-propanediol | - |
Homo sapiens | |
| 3.2.1.48 | 2-amino-2-methyl-1,3-propanediol | - |
Homo sapiens | |
| 3.2.1.48 | 2-amino-2-methyl-1-propanol | - |
Homo sapiens | |
| 3.2.1.48 | Bis-Tris | - |
Homo sapiens | |
| 3.2.1.48 | diethanolamine | - |
Homo sapiens | |
| 3.2.1.48 | Monoethanolamine | - |
Homo sapiens | |
| 3.2.1.48 | triethanolamine | - |
Homo sapiens | |
| 3.2.1.48 | Tris | concentration-dependent biphasic effect, first causing activation, fully competitive inhibition above pH 6.8, inhibition is stronger at alkaline pH values | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.10 | brush border | of the small intestinal absorptive cells of the villus and the crypts | Homo sapiens | 5903 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.10 | 130000 | - |
SDS-PAGE | Homo sapiens |
| 3.2.1.48 | 130000 | - |
gel filtration, density gradient, equilibrium centrifugation, sucrase subunit | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.10 | oligosaccharide + H2O | Homo sapiens | role in absorption of sucrose, isomaltose, maltose | monosaccharide | - |
? | |
| 3.2.1.48 | maltose + H2O | Homo sapiens | - |
2 glucose | - |
? | |
| 3.2.1.48 | sucrose + H2O | Homo sapiens | - |
D-glucose + D-fructose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.10 | Homo sapiens | - |
- |
- |
| 3.2.1.48 | Homo sapiens | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.10 | glycoprotein | - |
Homo sapiens |
| 3.2.1.10 | proteolytic modification | pro-sucrase-isomaltase precursor, complex glycosylated sucrase-isomaltase precursor, high-mannose sucrase-isomaltase precursor | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.10 | - |
Homo sapiens |
| 3.2.1.48 | - |
Homo sapiens |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.2.1.10 | alpha-D-glucopyranosyl-(1-4)-[alpha-D-glucopyranosyl-(1-6)]-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose + H2O = alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose + D-glucose | enzyme from intestinal mucosa also catalyzes the reaction of sucrase alpha-glucosidase, EC 3.2.1.48 | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.10 | small intestine | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.10 | 2.3 | - |
purified enzyme, isomaltose as substrate | Homo sapiens |
| 3.2.1.48 | 2.32 | - |
- |
Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.48 | -20°C | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.10 | isomaltosaccharides + H2O | n = 2, isomaltose | Homo sapiens | D-glucose | - |
? | |
| 3.2.1.10 | isomaltose + H2O | - |
Homo sapiens | 2 D-glucose | - |
? | |
| 3.2.1.10 | oligosaccharide + H2O | role in absorption of sucrose, isomaltose, maltose | Homo sapiens | monosaccharide | - |
? | |
| 3.2.1.48 | maltose + H2O | - |
Homo sapiens | 2 glucose | - |
? | |
| 3.2.1.48 | sucrose + H2O | - |
Homo sapiens | D-glucose + D-fructose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.10 | isomaltase | - |
Homo sapiens |
| 3.2.1.10 | sucrase-isomaltase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.10 | 37 | - |
- |
Homo sapiens |
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