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Literature summary extracted from

  • Quezada-Calvillo, R.; Markowitz, A.J.; Traber, P.G.; Underdown, B.J.
    Murine intestinal disaccharidases: identification of structural variants of sucrase-isomaltose complex (1993), Am. J. Physiol., 265, 1142-1149.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.48 mouse SI mRNA synthesized from mouse SI cDNA, pMSI-A1 cloned into pBluescript KS, cDNA cloned from mouse intestinal RNA with anchored polymerase chain reaction method Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.48 20.2 21.2 sucrose inbred strains of mice with genetic and posttranslational variations in SI complex structure, incubation at 37°C Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.48 220000
-
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.48 maltose + H2O Mus musculus
-
 2 glucose
-
 ?
3.2.1.48 sucrose + H2O Mus musculus
-
 D-glucose + D-fructose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.48 Mus musculus
-
 mouse, inbred strains CBA/J, CBA/Ca, CBA/CaH, CBA/CaHN, C57B1/6
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.48 17.5
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.48 maltose + H2O
-
 Mus musculus 2 glucose
-
 ?
3.2.1.48 sucrose + H2O
-
 Mus musculus D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.48 dimer
-
 Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.48 50
-
 pH 5.9, inactivation, soluble enzyme activity is stable to heat treatment, membrane associated enzyme was heat-labile under these conditions Mus musculus