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Literature summary extracted from

  • Muto, N.; Nakamura, T.; Yamamoto, I.
    Enzymatic formation of a nonreducing L-ascorbic acid alpha-glucoside: purification and properties of alpha-glucosidases catalyzing site-specific transglucosylation from rat small intestine (1990), J. Biochem., 107, 222-227.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 1.15
-
 maltose hydrolysis, alpha-glucosidase I Rattus norvegicus
3.2.1.20 1.9
-
 maltose transglucosylation, alpha-glucosidase II Rattus norvegicus
3.2.1.20 2.13
-
 maltose hydrolysis, alpha-glucosidase II Rattus norvegicus
3.2.1.20 2.98
-
 maltose transglucosylation, alpha-glucosidase I Rattus norvegicus
3.2.1.20 7.2
-
 L-ascorbic acid alpha-D-glucoside alpha-glucosidase I Rattus norvegicus
3.2.1.20 9.2
-
 L-ascorbic acid alpha-D-glucoside alpha-glucosidase II Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 membrane bound Rattus norvegicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 270000
-
 alpha-glucosidase II, gel filtration Rattus norvegicus
3.2.1.20 560000
-
 alpha-glucosidase I, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.48 maltose + H2O Rattus rattus
-
 2 glucose
-
 ?
3.2.1.48 sucrose + H2O Rattus rattus
-
 D-glucose + D-fructose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Rattus norvegicus
-
-
-
3.2.1.48 Rattus rattus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20
-
 Rattus norvegicus
3.2.1.48
-
 Rattus rattus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 intestine small intestine Rattus norvegicus
-
3.2.1.20 intestine microvillus Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 23.6
-
 alpha-glucosidase II Rattus norvegicus
3.2.1.20 60.6
-
 alpha-glucosidase I Rattus norvegicus
3.2.1.48 9.5
-
 maltose to L-ascorbic acid alpha-glucoside forming activity Rattus rattus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 L-ascorbic acid alpha-D-glucoside
-
 Rattus norvegicus maltose + L-ascorbic acid
-
 r
3.2.1.20 L-ascorbic acid alpha-D-glucoside + H2O
-
 Rattus norvegicus maltose + L-ascorbic acid
-
 r
3.2.1.20 maltose + L-ascorbic acid
-
 Rattus norvegicus L-ascorbic acid alpha-D-glucoside
-
 r
3.2.1.48 L-ascorbic acid alpha-glucoside + H2O
-
 Rattus rattus ?
-
 ?
3.2.1.48 maltose + H2O
-
 Rattus rattus 2 glucose
-
 ?
3.2.1.48 maltose + L-ascorbic acid
-
 Rattus rattus L-ascorbic acid alpha-D-glucoside enzyme form L-ascorbic acid alpha-glucoside by splitting maltose among the disaccharides r
3.2.1.48 sucrose + H2O
-
 Rattus rattus D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 More 3 bands detected by SDS-PAGE of alpha-glucosidase I: 130000 Da, 210000 Da and 330000 Da. A major band of 135000 Da and a minor band of 145000 Da is detected by SDS-PAGE of alpha-glucosidase II Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 60
-
-
 Rattus norvegicus
3.2.1.48 60
-
 maximal activity of L-ascorbic acid alpha-glucoside formation and maltose hydrolysis Rattus rattus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 4.5 5 hydrolysis of maltose Rattus norvegicus
3.2.1.20 6 6.5 formation of L-ascorbic acid alpha-glucoside Rattus norvegicus
3.2.1.48 4.5 5 L-ascorbic acid alpha-glucoside formation Rattus rattus