Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sjöström, H.; Noren, O.; Christiansen, L.; Wacker, H.; Semenza, G.
    A fully active, two-active-site, single-chain sucrase-isomaltase from pig small intestine. Implications for the biosynthesis of a mammalian integral stalked membrane protein (1980), J. Biol. Chem., 255, 11332-11338.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.10 chymotrypsin almost 25% of palatinase activity after incubation with 0.1 mg/ml for 30 min Sus scrofa
3.2.1.10 Elastase almost 50% of palatinase activity after incubation with 0.1 mg/ml for 30 min Sus scrofa
3.2.1.10 Trypsin almost 25% of palatinase activity after incubation with 0.1 mg/ml for 30 min Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.10 140000 150000 SDS-PAGE, intact pancreatic duct, 2 polypeptide chains, 140000 + 150000 Sus scrofa
3.2.1.10 150000
-
 isomaltase in ileum, SDS-PAGE Sus scrofa
3.2.1.10 260000
-
 SDS-PAGE, pancreas disconnected from duodenum, single polypeptide chain Sus scrofa
3.2.1.48 140000
-
 SDS-PAGE, intact pancreatic duct, subunit I Sus scrofa
3.2.1.48 150000
-
 SDS-PAGE, intact pancreatic duct, subunit II Sus scrofa
3.2.1.48 260000
-
 SDS-PAGE, disconnected pancreatic duct Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.48 maltose + H2O Sus scrofa
-
 2 glucose
-
 ?
3.2.1.48 sucrose + H2O Sus scrofa
-
 D-glucose + D-fructose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.10 Sus scrofa
-
-
-
3.2.1.48 Sus scrofa
-
 pig
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.10 proteolytic modification extracellular proteolysis of single-chain pro-sucrase-isomaltase Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.10
-
 Sus scrofa
3.2.1.48
-
 Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.10 alpha-D-glucopyranosyl-(1-4)-[alpha-D-glucopyranosyl-(1-6)]-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose + H2O = alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose + D-glucose enzyme from intestinal mucosa also catalyzes the reaction of sucrase alpha-glucosidase, EC 3.2.1.48 Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.10 small intestine ileum Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.48 additional information
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.10 maltose + H2O
-
 Sus scrofa 2 D-glucose
-
 ?
3.2.1.10 palatinose + H2O
-
 Sus scrofa D-glucose + fructose
-
 ?
3.2.1.48 maltose + H2O
-
 Sus scrofa 2 glucose
-
 ?
3.2.1.48 sucrose + H2O
-
 Sus scrofa D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.48 dimer 1 * 140000 + 1 * 150000, intact pancreatic duct, SDS -PAGE Sus scrofa
3.2.1.48 monomer disconnected pancreatic duct, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.10 sucrase-isomaltase consisting of two polypeptid chains Sus scrofa