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Literature summary extracted from

  • Rudiger, A.; Jorgensen, P.L.; Antranikian, G.
    Isolation and characterization of a heat-stable pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after cloning and expression of its gene in Escherichia coli (1995), Appl. Environ. Microbiol., 61, 567-575.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.41 2-mercaptoethanol at 10 mM Pyrococcus woesei
3.2.1.41 dithiothreitol at 10 mM Pyrococcus woesei
3.2.1.41 Triton X-100 at 0.1% Pyrococcus woesei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41 expression in Escherichia coli Pyrococcus woesei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 Cr3+
-
 Pyrococcus woesei
3.2.1.41 Cu2+
-
 Pyrococcus woesei
3.2.1.41 Cyclodextrins
-
 Pyrococcus woesei
3.2.1.41 Fe2+
-
 Pyrococcus woesei
3.2.1.41 Guanidine HCl at 0.25 M Pyrococcus woesei
3.2.1.41 iodoacetamide at 10 mM Pyrococcus woesei
3.2.1.41 N-bromosuccinimide
-
 Pyrococcus woesei
3.2.1.41 Ni2+
-
 Pyrococcus woesei
3.2.1.41 Sodium dodecyl sulfate
-
 Pyrococcus woesei
3.2.1.41 Urea at 6 M Pyrococcus woesei
3.2.1.41 Zn2+
-
 Pyrococcus woesei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.41 Ca2+ highly stimulated Pyrococcus woesei
3.2.1.41 Co2+
-
 Pyrococcus woesei
3.2.1.41 Mn2+ slightly stimulated Pyrococcus woesei
3.2.1.41 Na2MoO4
-
 Pyrococcus woesei
3.2.1.41 Na2WO4
-
 Pyrococcus woesei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 90000
-
 gel electrophoresis Pyrococcus woesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.41 amylopectin + H2O Pyrococcus woesei branched polysaccharides maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O Pyrococcus woesei
-
 maltotriose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Pyrococcus woesei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41 of the recombinant protein Pyrococcus woesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
 Pyrococcus woesei maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O branched polysaccharides Pyrococcus woesei maltooligosaccharides + maltose
-
 ?
3.2.1.41 dextrin + H2O
-
 Pyrococcus woesei maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O
-
 Pyrococcus woesei maltotriose + ?
-
 ?
3.2.1.41 soluble starch
-
 Pyrococcus woesei maltotriose + maltose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 amylase-pullulanase
-
 Pyrococcus woesei
3.2.1.41 pullulanase type II hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides Pyrococcus woesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 100
-
-
 Pyrococcus woesei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.41 80 120 40% activity at 120°C Pyrococcus woesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 100
-
 after 4 h, 15% loss of activity Pyrococcus woesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 6
-
-
 Pyrococcus woesei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.41 5.5 9
-
 Pyrococcus woesei