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Literature summary extracted from

  • Kambourova, M.S.; Emanuilova, E.I.
    Purification and general biochemical properties of thermostable pullulanase from Bacillus stearothermophilus G-82 (1992), Appl. Biochem. Biotechnol., 33, 193-203.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 1,10-phenanthroline
-
Geobacillus stearothermophilus
3.2.1.41 Ca2+
-
Geobacillus stearothermophilus
3.2.1.41 Co2+
-
Geobacillus stearothermophilus
3.2.1.41 Cu2+
-
Geobacillus stearothermophilus
3.2.1.41 Fe3+
-
Geobacillus stearothermophilus
3.2.1.41 Mg2+
-
Geobacillus stearothermophilus
3.2.1.41 Mn2+
-
Geobacillus stearothermophilus
3.2.1.41 N-bromosuccinimide
-
Geobacillus stearothermophilus
3.2.1.41 phenylmethylsulfonyl fluoride partially Geobacillus stearothermophilus
3.2.1.41 Zn2+
-
Geobacillus stearothermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.41 extracellular
-
Geobacillus stearothermophilus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 53000 56000 gel filtration, gel electrophoresis Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.41 amylopectin + H2O Geobacillus stearothermophilus branched polysaccharides maltooligosaccharides + maltose
-
?
3.2.1.41 pullulan + H2O Geobacillus stearothermophilus
-
maltotriose + ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41 to homogeneity Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
Geobacillus stearothermophilus maltooligosaccharides + maltose
-
?
3.2.1.41 amylopectin + H2O branched polysaccharides Geobacillus stearothermophilus maltooligosaccharides + maltose
-
?
3.2.1.41 amylose + H2O weakly Geobacillus stearothermophilus maltooligosaccharides + maltose
-
?
3.2.1.41 dextrin + H2O
-
Geobacillus stearothermophilus maltooligosaccharides + maltose
-
?
3.2.1.41 glycogen + H2O
-
Geobacillus stearothermophilus maltooligosaccharides + maltose
-
?
3.2.1.41 maltoheptaose + H2O
-
Geobacillus stearothermophilus maltotriose + maltose + D-glucose
-
?
3.2.1.41 maltohexaose + H2O
-
Geobacillus stearothermophilus maltotriose + maltose + D-glucose + maltotetraose
-
?
3.2.1.41 maltopentaose + H2O
-
Geobacillus stearothermophilus maltotriose + maltose
-
?
3.2.1.41 maltotetraose + H2O
-
Geobacillus stearothermophilus maltose + glucose + maltotriose
-
?
3.2.1.41 pullulan + H2O
-
Geobacillus stearothermophilus maltotriose + ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 pullulanase type II hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 60 65
-
Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.41 25 75
-
Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 60
-
no loss of activity after 10 min Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 7
-
-
Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.41 4.5 9
-
Geobacillus stearothermophilus