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Literature summary extracted from

  • Ohba, R.; Ueda, S.
    Some properties of crystalline extra- and intracellular pullulanases from Aerobacter aerogenes (1975), Agric. Biol. Chem., 39, 967-972.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.41 ammonium sulfate precipitation Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 Al3+
-
 Klebsiella aerogenes
3.2.1.41 Cd2+
-
 Klebsiella aerogenes
3.2.1.41 Co2+
-
 Klebsiella aerogenes
3.2.1.41 Cu2+
-
 Klebsiella aerogenes
3.2.1.41 EDTA
-
 Klebsiella aerogenes
3.2.1.41 Fe2+
-
 Klebsiella aerogenes
3.2.1.41 Fe3+
-
 Klebsiella aerogenes
3.2.1.41 Hg2+
-
 Klebsiella aerogenes
3.2.1.41 N-bromosuccinimide
-
 Klebsiella aerogenes
3.2.1.41 Ni2+
-
 Klebsiella aerogenes
3.2.1.41 p-chloromercuribenzoate
-
 Klebsiella aerogenes
3.2.1.41 Pb2+
-
 Klebsiella aerogenes
3.2.1.41 Sodium dodecyl sulfate
-
 Klebsiella aerogenes
3.2.1.41 Zn2+
-
 Klebsiella aerogenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.41 cytoplasm
-
 Klebsiella aerogenes 5737
-
3.2.1.41 extracellular
-
 Klebsiella aerogenes
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.41 Ca2+
-
 Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.41 amylopectin + H2O Klebsiella aerogenes branched polysaccharides maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O Klebsiella aerogenes
-
 maltotriose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Klebsiella aerogenes
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
 Klebsiella aerogenes maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O branched polysaccharides Klebsiella aerogenes maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O
-
 Klebsiella aerogenes maltotriose + ?
-
 ?
3.2.1.41 soluble starch
-
 Klebsiella aerogenes maltotriose + maltose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 pullulanase type II hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 6
-
-
 Klebsiella aerogenes