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Literature summary extracted from
- Formation, separation and characterization of three beta-1,3-glucanases from Scleroticum glucanicum (1992), Biochim. Biophys. Acta, 1117, 7-14.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.39 | Hg2+ | inhibition at 1 mM | Sclerotium glucanicum |  |
| 3.2.1.39 | p-hydroxymercuribenzoate | strong inhibition at 1 mM | Sclerotium glucanicum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.39 | additional information | - |
Laminarin | value in mg/ml | Sclerotium glucanicum | |
| 3.2.1.39 | additional information | - |
Laminarin | 2.5 | Sclerotium glucanicum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.39 | extracellular | - |
Sclerotium glucanicum | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.39 | 85000 | - |
SDS-PAGE | Sclerotium glucanicum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.39 | Sclerotium glucanicum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.39 | to homogeneity, 1step chromatography | Sclerotium glucanicum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.39 | culture filtrate | - |
Sclerotium glucanicum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.39 | 69 | - |
- |
Sclerotium glucanicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.39 | laminarin + H2O | specifically hydrolyzes beta-1,3-bonds, final products are glucose, laminaribiose and laminaritetraose | Sclerotium glucanicum | D-glucose + ? | - |
? | |
| 3.2.1.39 | scleroglucan + H2O | low activity | Sclerotium glucanicum | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.39 | 55 | - |
- |
Sclerotium glucanicum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.39 | 55 | - |
63% activity after 10 min | Sclerotium glucanicum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.39 | 4.7 | - |
- |
Sclerotium glucanicum |
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Release 2023.1 (January 2023)
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