Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chinchetru, M.A.; Cabezas, J.A.; Calvo, P.
    Characterization and kinetics of beta-D-gluco/fuco/galactosidase from sheep liver (1983), Comp. Biochem. Physiol. B, 75, 719-728.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.38 stable against repeated freezing and thawing cycles, activity unstable in SDS Ovis aries aries

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.38 4-O-alpha-D-glucopyranosyl-D-glucose effective inhibitor at 50 mM Ovis aries aries
3.2.1.38 beta-D-fucose strong competitive inhibition Ovis aries aries
3.2.1.38 beta-D-galactose competitive inhibition Ovis aries aries
3.2.1.38 beta-D-glucose weak competitive inhibition Ovis aries aries
3.2.1.38 D-fucono-gamma-lactone effective inhibition at 50 mM Ovis aries aries
3.2.1.38 glucono 1,5-lactone effective inhibition at 50 mM Ovis aries aries
3.2.1.38 Hg2+ effective inhibitor at 10 mM Ovis aries aries
3.2.1.38 KCN effective inhibitor at 10 mM Ovis aries aries
3.2.1.38 Tris effective inhibitor at 10 mM Ovis aries aries
3.2.1.38 Urea lowers beta-D-glucosidase and beta-D-fucosidase activities, not beta-D-galactosidase activity Ovis aries aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.38 0.2
-
 p-nitrophenyl-beta-D-glucoside
-
 Ovis aries aries
3.2.1.38 0.25
-
 p-nitrophenyl-beta-D-galactoside
-
 Ovis aries aries
3.2.1.38 0.33
-
 p-nitrophenyl-beta-D-fucoside
-
 Ovis aries aries

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.38 Ovis aries aries
-
 sheep
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.38 to homogeneity by several chromatography steps, isoelectric focusing Ovis aries aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.38 liver
-
 Ovis aries aries
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.38 p-nitrophenyl-beta-D-fucoside + H2O common active site for beta-D-fucosidase and beta-D-glucosidase, transglycosylation activity, independent active site for beta-D-galactosidase Ovis aries aries p-nitrophenol + beta-D-fucose
-
 ?
3.2.1.38 p-nitrophenyl-beta-D-fucoside + H2O contains a single active site for hydrolysis of both p-nitrophenyl-beta-D-fucoside and p-nitrophenyl-beta-D-glucoside Ovis aries aries p-nitrophenol + beta-D-fucose
-
 ?
3.2.1.38 p-nitrophenyl-beta-D-galactoside + H2O
-
 Ovis aries aries p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.38 p-nitrophenyl-beta-D-glucoside + H2O contains a single active site for hydrolysis of both p-nitrophenyl-beta-D-glucoside and p-nitrophenyl-beta-D-fucoside Ovis aries aries p-nitrophenol + beta-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.38 beta-D-gluco/fuco/galactosidase
-
 Ovis aries aries

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.38 40
-
 not stable above Ovis aries aries

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.38 4.5 5.5
-
 Ovis aries aries

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.38 5.5 7 beta-D-fucosidase, beta-D-glucosidase, beta-D-galactosidase activity Ovis aries aries