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Literature summary extracted from

  • Srisomsap, C.; Svasti, J.; Surarit, R.; Champattanachai, V.; Sawangareetrakul, P.; Boonpuan, K.; Subhasitanont, P.; Chokchaichamnankit, D.
    Isolation and characterization of an enzyme with beta-glucosidase and beta-fucosidase activities from Dalbergia cochinchinensis Pierre (1996), J. Biochem., 119, 585-590.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.38 glucono 1,5-lactone inhibitory between 0.1 and 1 mM Dalbergia cochinchinensis
3.2.1.38 Hg2+ strong inhibition at 0.001 mM Dalbergia cochinchinensis
3.2.1.38 p-chloromercuribenzoate strong inhibition between 0.01 mM and 0.001 mM Dalbergia cochinchinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.38 0.54
-
 p-nitrophenyl-beta-D-fucoside
-
 Dalbergia cochinchinensis
3.2.1.38 5.37
-
 p-nitrophenyl-beta-D-glucoside
-
 Dalbergia cochinchinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.38 66000
-
 SDS-PAGE Dalbergia cochinchinensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.38 Dalbergia cochinchinensis
-
 thai rosewood
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.38 to homogeneity by isoelectric focusing and chromatography steps Dalbergia cochinchinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.38 seed highest level compared to other tissues Dalbergia cochinchinensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.38 48
-
 for beta-D-glucosidase activity Dalbergia cochinchinensis
3.2.1.38 62
-
 for beta-D-fucosidase activity Dalbergia cochinchinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.38 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin, low activity Dalbergia cochinchinensis 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.38 (R)-alpha-[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]benzeneacetonitrile + H2O amygdalin, low activity Dalbergia cochinchinensis ?
-
 ?
3.2.1.38 p-nitrophenyl-alpha-L-arabinoside + H2O low activity Dalbergia cochinchinensis p-nitrophenol + alpha-L-arabinose
-
 ?
3.2.1.38 p-nitrophenyl-beta-D-fucoside + H2O high activity Dalbergia cochinchinensis p-nitrophenol + beta-D-fucose
-
 ?
3.2.1.38 p-nitrophenyl-beta-D-galactoside + H2O low activity Dalbergia cochinchinensis p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.38 p-nitrophenyl-beta-D-glucoside + H2O high activity Dalbergia cochinchinensis p-nitrophenol + beta-D-glucose
-
 ?
3.2.1.38 p-nitrophenyl-beta-D-xyloside + H2O low activity Dalbergia cochinchinensis p-nitrophenol + beta-D-xylose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.38 5
-
-
 Dalbergia cochinchinensis