Any feedback?
Literature summary extracted from
- Effective induction, purification and characterization of Trichoderma koningii G-39 beta-xylosidase with high transferase activity (2000), Biotechnol. Appl. Biochem., 31, 119-125.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.37 | synthesis | synthesis of a group of uncommon xylosides via the transxylosylation activity | Trichoderma koningii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.37 | beta-D-xylose | moderate product inhibition, Ki: 5 mM | Trichoderma koningii |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.37 | 0.02 | - |
2,4-dinitrophenyl beta-D-xylopyranoside | - |
Trichoderma koningii | |
| 3.2.1.37 | 0.02 | - |
4-chloro-2-nitrophenyl beta-D-xylopyranoside | - |
Trichoderma koningii | |
| 3.2.1.37 | 0.03 | - |
o-nitrophenyl beta-D-xylopyranoside | - |
Trichoderma koningii | |
| 3.2.1.37 | 0.04 | - |
p-nitrophenyl beta-D-xylopyranoside | - |
Trichoderma koningii | |
| 3.2.1.37 | 7.5 | - |
p-nitrophenyl-alpha-L-arabinopyranoside | - |
Trichoderma koningii | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.37 | extracellular | - |
Trichoderma koningii | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 100000 | 105000 | gel filtration, SDS-PAGE | Trichoderma koningii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.37 | xylo-oligosaccharides + H2O | Trichoderma koningii | derived from xylan | beta-D-xylose + ? | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.2.1.37 | acetonitrile | stable in the presence of 20%, v/v | Trichoderma koningii |
| 3.2.1.37 | Methanol | stable in the presence of alcohols, 20%, v/v | Trichoderma koningii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.37 | Trichoderma koningii | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.37 | glycoprotein | - |
Trichoderma koningii |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.37 | near homogeneity, anion exchange chromatography | Trichoderma koningii |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.37 | mycelium | induced enzyme production by addition of 1% xylan and 0.1% xylose in the medium | Trichoderma koningii | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 3.02 | - |
- |
Trichoderma koningii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.37 | 2,4-dinitrophenyl beta-D-xylopyranoside + H2O | broad tolerance for the aglycone portion of the substrate | Trichoderma koningii | 2,4-dinitrophenol + beta-D-xylopyranose | - |
? | |
| 3.2.1.37 | 4-chloro-2-nitrophenyl beta-D-xylopyranoside + H2O | - |
Trichoderma koningii | 4-chloro-2-nitrophenol + beta-D-xylopyranose | - |
? | |
| 3.2.1.37 | 4-nitrophenyl beta-D-xylopyranoside + methanol | transxylosylation activity, NMR study in the presence of 15% methanol, v/v | Trichoderma koningii | 4-nitrophenol + methyl beta-D-xylopyranoside | very weak peaks of D-xylose-alpha-anomers, most D-xylose-beta-anomers | ? | |
| 3.2.1.37 | o-nitrophenyl beta-D-xylopyranoside + H2O | - |
Trichoderma koningii | o-nitrophenol + beta-D-xylopyranose | - |
? | |
| 3.2.1.37 | p-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Trichoderma koningii | p-nitrophenol + alpha-L-arabinopyranose | - |
? | |
| 3.2.1.37 | p-nitrophenyl beta-D-xylopyranoside + H2O | - |
Trichoderma koningii | p-nitrophenol + beta-D-xylopyranose | - |
? | |
| 3.2.1.37 | xylo-oligosaccharides + H2O | derived from xylan | Trichoderma koningii | beta-D-xylose + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.37 | monomer | 1 * 100000-105000, gel filtration, SDS-PAGE | Trichoderma koningii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 60 | - |
- |
Trichoderma koningii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 60 | - |
quite stable below | Trichoderma koningii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 3.5 | 4 | - |
Trichoderma koningii |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.37 | 2.5 | 7.4 | at 37°C, very unstable below pH 1.7 and above pH 9.5 | Trichoderma koningii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
