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Literature summary extracted from

  • Fierobe, H.P.; Clarke, A.J.; Tull, D.; Svensson, B.
    Enzymatic properties of the cysteinesulfinic acid derivative of the catalytic-base mutant Glu400->Cys of glucoamylase from Aspergillus awamori (1998), Biochemistry, 37, 3753-3759.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.3 E400C cysteinesulfinic acid derivative of C320A/E400C-SO2H has a 700times higher turnover number towards maltose relative to C320A/E400C, while the Km-value is unchanged. Compared to wild-type enzyme, the C400-SO2H derivative has a turnover number of 150-190% and 85-320% on maltooliogosaccharides and isomaltooligosaccharides respectively, while Km-values are similar to that of wild-type for disaccharides and 3.5-5.5fold and 1.8-2.5fold higher for the longer maltooligosaccharides and isomaltooligosaccharides. The inhibition constant of cysteinesulfinic acid derivative of C320A/E400C-SO2H for acarbose increases more than 10000-fold Aspergillus awamori

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 1-deoxynojirimycin
-
 Aspergillus awamori
3.2.1.3 acarbose
-
 Aspergillus awamori
3.2.1.3 beta-O-methylacarviosinide
-
 Aspergillus awamori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information Km-values for C320A/E400C and the cysteinesulfinic acid derivative of C320A/E400C Aspergillus awamori
3.2.1.3 0.1
-
 maltopentaose wild-type enzyme Aspergillus awamori
3.2.1.3 0.11
-
 maltohexaose wild-type enzyme Aspergillus awamori
3.2.1.3 0.12
-
 maltoheptaose wild-type enzyme Aspergillus awamori
3.2.1.3 0.18
-
 maltotetraose wild-type enzyme Aspergillus awamori
3.2.1.3 0.29
-
 maltotriose wild-type enzyme Aspergillus awamori
3.2.1.3 3
-
 maltose wild-type enzyme Aspergillus awamori
3.2.1.3 3.4
-
 4-nitrophenyl alpha-D-glucoside wild-type enzyme Aspergillus awamori
3.2.1.3 17
-
 panose wild-type enzyme Aspergillus awamori
3.2.1.3 32
-
 nigerose wild-type enzyme Aspergillus awamori
3.2.1.3 38
-
 isomaltose wild-type enzyme Aspergillus awamori
3.2.1.3 70
-
 alpha-D-glucopyranosyl fluoride wild-type enzyme Aspergillus awamori
3.2.1.3 150
-
 kojibiose wild-type enzyme Aspergillus awamori

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Aspergillus awamori
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 4-nitrophenyl alpha-D-glucoside + H2O
-
 Aspergillus awamori 4-nitrophenol + D-glucose
-
 ?
3.2.1.3 alpha-alpha-trehalose + H2O
-
 Aspergillus awamori alpha-D-glucose
-
 ?
3.2.1.3 alpha-D-glucopyranosyl fluoride + H2O
-
 Aspergillus awamori ?
-
 ?
3.2.1.3 amylopectin + H2O
-
 Aspergillus awamori D-glucose + ?
-
 ?
3.2.1.3 glycogen + H2O
-
 Aspergillus awamori glucose + ?
-
 ?
3.2.1.3 isomaltose + H2O
-
 Aspergillus awamori glucose
-
 ?
3.2.1.3 kojibiose + H2O
-
 Aspergillus awamori alpha-D-glucose + D-glucose
-
 ?
3.2.1.3 maltoheptaose + H2O
-
 Aspergillus awamori ?
-
 ?
3.2.1.3 maltohexaose + H2O
-
 Aspergillus awamori ?
-
 ?
3.2.1.3 maltopentaose + H2O
-
 Aspergillus awamori ?
-
 ?
3.2.1.3 maltose + H2O
-
 Aspergillus awamori 2 glucose
-
 ?
3.2.1.3 maltotetraose + H2O
-
 Aspergillus awamori ?
-
 ?
3.2.1.3 maltotriose + H2O
-
 Aspergillus awamori maltose + glucose
-
 ?
3.2.1.3 nigerose + H2O
-
 Aspergillus awamori 2 alpha-D-glucose
-
 ?
3.2.1.3 panose + H2O
-
 Aspergillus awamori ?
-
 ?
3.2.1.3 starch + H2O soluble Aspergillus awamori glucose + ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information turnover numbers for C320A/E400C and the cysteinesulfinic acid derivative of C320A/E400C Aspergillus awamori
3.2.1.3 0.13
-
 kojibiose wild-type enzyme Aspergillus awamori
3.2.1.3 0.14
-
 nigerose wild-type enzyme Aspergillus awamori
3.2.1.3 0.25
-
 4-nitrophenyl alpha-D-glucoside wild-type enzyme Aspergillus awamori
3.2.1.3 0.27
-
 isomaltose wild-type enzyme Aspergillus awamori
3.2.1.3 6.8
-
 maltose wild-type enzyme Aspergillus awamori
3.2.1.3 6.9
-
 panose wild-type enzyme Aspergillus awamori
3.2.1.3 27.3
-
 maltopentaose wild-type enzyme Aspergillus awamori
3.2.1.3 28
-
 maltotriose wild-type enzyme Aspergillus awamori
3.2.1.3 45
-
 maltotetraose wild-type enzyme Aspergillus awamori
3.2.1.3 49
-
 maltohexaose wild-type enzyme Aspergillus awamori
3.2.1.3 54
-
 maltoheptaose wild-type enzyme Aspergillus awamori
3.2.1.3 300
-
 alpha-D-glucopyranosyl fluoride wild-type enzyme Aspergillus awamori