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Literature summary extracted from

  • Abe, J.I.; Nagano, H.; Hizukuri, S.
    Kinetic and structural properties of the three forms of glucoamylase of Rhizopus delemar (1985), J. Appl. Biochem., 7, 235-247.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 0.09
-
 maltoheptaose
-
 Aspergillus niger
3.2.1.3 0.1
-
 maltohexaose
-
 Aspergillus niger
3.2.1.3 0.1
-
 maltoheptaose enzyme form GII Rhizopus arrhizus
3.2.1.3 0.11
-
 maltohexaose enzyme form GII Rhizopus arrhizus
3.2.1.3 0.11
-
 maltoheptaose enzyme form GI Rhizopus arrhizus
3.2.1.3 0.12
-
 maltopentaose
-
 Aspergillus niger
3.2.1.3 0.12
-
 maltohexaose enzyme form GI Rhizopus arrhizus
3.2.1.3 0.14
-
 maltotetraose
-
 Aspergillus niger
3.2.1.3 0.14
-
 maltopentaose enzyme form GII Rhizopus arrhizus
3.2.1.3 0.15
-
 maltoheptaose
-
 Aspergillus niger
3.2.1.3 0.15
-
 maltoheptaose enzyme form GIII Rhizopus arrhizus
3.2.1.3 0.16
-
 maltopentaose enzyme form GI Rhizopus arrhizus
3.2.1.3 0.16
-
 maltohexaose enzyme form GIII Rhizopus arrhizus
3.2.1.3 0.17
-
 maltopentaose enzyme form GIII Rhizopus arrhizus
3.2.1.3 0.21
-
 maltotetraose enzyme form GII Rhizopus arrhizus
3.2.1.3 0.23
-
 maltotetraose enzyme form GI Rhizopus arrhizus
3.2.1.3 0.26
-
 maltotetraose enzyme form GIII Rhizopus arrhizus
3.2.1.3 0.3
-
 maltotriose
-
 Aspergillus niger
3.2.1.3 0.3
-
 amylopectin enzyme form GIII Rhizopus arrhizus
3.2.1.3 0.57
-
 maltotriose enzyme form GII Rhizopus arrhizus
3.2.1.3 0.59
-
 maltotriose enzyme form GI Rhizopus arrhizus
3.2.1.3 0.64
-
 maltotriose enzyme form GIII Rhizopus arrhizus
3.2.1.3 0.8
-
 glycogen glycogen from rabbit liver, enzyme form GIII Rhizopus arrhizus
3.2.1.3 0.91
-
 maltose
-
 Aspergillus niger
3.2.1.3 1.8
-
 glycogen glycogen from oyster, enzyme form GIII Rhizopus arrhizus
3.2.1.3 1.9
-
 maltose enzyme form GI or GII Rhizopus arrhizus
3.2.1.3 2
-
 maltose enzyme form GIII Rhizopus arrhizus
3.2.1.3 6.1
-
 amylopectin enzyme form GI Rhizopus arrhizus
3.2.1.3 7.4
-
 amylopectin enzyme form GII Rhizopus arrhizus
3.2.1.3 13
-
 panose
-
 Aspergillus niger
3.2.1.3 13
-
 panose enzyme form GII Rhizopus arrhizus
3.2.1.3 14
-
 panose enzyme form GI or GIII Rhizopus arrhizus
3.2.1.3 18
-
 pullulan enzyme form GIII Rhizopus arrhizus
3.2.1.3 40
-
 isomaltose enzyme form GIII Rhizopus arrhizus
3.2.1.3 42
-
 isomaltose
-
 Aspergillus niger
3.2.1.3 47
-
 isomaltose enzyme form GI or GII Rhizopus arrhizus
3.2.1.3 59
-
 amylopectin
-
 Aspergillus niger
3.2.1.3 210
-
 glycogen glycogen from oyster, enzyme form GI Rhizopus arrhizus
3.2.1.3 240
-
 glycogen glycogen from rabbit liver, enzyme form GI Rhizopus arrhizus
3.2.1.3 250
-
 glycogen glycogen from oyster, enzyme form GII Rhizopus arrhizus
3.2.1.3 300
-
 glycogen glycogen from rabbit liver, enzyme form GII Rhizopus arrhizus
3.2.1.3 820
-
 glycogen glycogen from rabbit liver Aspergillus niger
3.2.1.3 1100
-
 glycogen glycogen from oyster Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 61000
-
 x * 61000, enzyme form GI, SDS-PAGE Rhizopus arrhizus
3.2.1.3 70000
-
 x * 70000, enzyme form GII, SDS-PAGE Rhizopus arrhizus
3.2.1.3 78000
-
 x * 78000, enzyme form GIII, SDS-PAGE Rhizopus arrhizus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Aspergillus niger
-
-
-
3.2.1.3 Rhizopus arrhizus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein
-
 Aspergillus niger
3.2.1.3 glycoprotein
-
 Rhizopus arrhizus
3.2.1.3 proteolytic modification
-
 Aspergillus niger
3.2.1.3 proteolytic modification enzyme form GI and GII are transformed products of enzyme form GIII due to the limited action of proteases and glucosidases. GII is formed from GIII upon the release of a peptide portion of approximately 8000 Da. Enzyme form GI is formed from enzyme form GII and GIII on the removal of both peptide and carbohydrate chains and/or glycopeptide fragments Rhizopus arrhizus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 3 enzyme forms: GI, GII and GIII Rhizopus arrhizus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 additional information
-
-
 Aspergillus niger
3.2.1.3 additional information
-
-
 Rhizopus arrhizus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 amylopectin + H2O
-
 Aspergillus niger D-glucose + ?
-
 ?
3.2.1.3 amylopectin + H2O
-
 Rhizopus arrhizus D-glucose + ?
-
 ?
3.2.1.3 glycogen + H2O
-
 Aspergillus niger glucose + ?
-
 ?
3.2.1.3 glycogen + H2O
-
 Rhizopus arrhizus beta-D-glucose + glycogen
-
 ?
3.2.1.3 isomaltose + H2O
-
 Aspergillus niger glucose
-
 ?
3.2.1.3 isomaltose + H2O
-
 Rhizopus arrhizus glucose
-
 ?
3.2.1.3 maltoheptaose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 maltoheptaose + H2O
-
 Rhizopus arrhizus ?
-
 ?
3.2.1.3 maltohexaose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 maltohexaose + H2O
-
 Rhizopus arrhizus ?
-
 ?
3.2.1.3 maltopentaose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 maltopentaose + H2O
-
 Rhizopus arrhizus ?
-
 ?
3.2.1.3 maltose + H2O
-
 Aspergillus niger 2 glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Rhizopus arrhizus 2 glucose
-
 ?
3.2.1.3 maltotetraose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 maltotetraose + H2O
-
 Rhizopus arrhizus ?
-
 ?
3.2.1.3 maltotriose + H2O
-
 Aspergillus niger maltose + glucose
-
 ?
3.2.1.3 maltotriose + H2O
-
 Rhizopus arrhizus maltose + glucose
-
 ?
3.2.1.3 panose + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.3 panose + H2O
-
 Rhizopus arrhizus ?
-
 ?
3.2.1.3 pullulan + H2O
-
 Rhizopus arrhizus beta-D-glucose + pullulan
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 ? x * 70000, enzyme form GII, SDS-PAGE Rhizopus arrhizus
3.2.1.3 ? x * 78000, enzyme form GIII, SDS-PAGE Rhizopus arrhizus
3.2.1.3 ? x * 61000, enzyme form GI, SDS-PAGE Rhizopus arrhizus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 45
-
 pH 4.5, 10 min, quite stable up to Rhizopus arrhizus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.3 4 8.5 40°C, 30 min, stable Rhizopus arrhizus