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Literature summary extracted from

  • Nakano, H.; Moriwaki, M.; Washino, T.; Kino, T.; Yoshizumi, H.; Kitahara, S.
    Purification and some properties of a trehalase from a green alga, Lobosphaera sp. (1994), Biosci. Biotechnol. Biochem., 58, 1430-1434.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 Cu2+ weak inhibition Lobosphaera sp.
3.2.1.28 Fe3+
-
 Lobosphaera sp.
3.2.1.28 Mn2+ weak Lobosphaera sp.
3.2.1.28 sucrose 25 mM, 33% inhibition; competitive Lobosphaera sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 0.6
-
 trehalose
-
 Lobosphaera sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 intracellular
-
 Lobosphaera sp. 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 400000
-
 gel filtration Lobosphaera sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Lobosphaera sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.28 glycoprotein
-
 Lobosphaera sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28
-
 Lobosphaera sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 additional information
-
-
 Lobosphaera sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 trehalose + H2O
-
 Lobosphaera sp. D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 dimer 2 * 180000-220000, SDS-PAGE Lobosphaera sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 65
-
-
 Lobosphaera sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.28 65
-
 10 min, pH 5.5, stable below Lobosphaera sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 5.5
-
-
 Lobosphaera sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.28 4 9 5°C, 15 h, stable Lobosphaera sp.