EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.28 | - |
Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.28 | K+ | 0.5 M KCl, activity is 2fold lower | Escherichia coli | |
3.2.1.28 | Na+ | 0.5 M NaCl, activity is 2fold lower | Escherichia coli | |
3.2.1.28 | potassium glutamate | 0.5 M KCl, activity is 2fold lower | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.28 | 1.9 | - |
trehalose | cytoplasmic enzyme | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.28 | cytoplasm | - |
Escherichia coli | 5737 | - |
3.2.1.28 | periplasm | at high osmolarity a periplasmic trehalase is induced | Escherichia coli | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.28 | additional information | cytoplasmic enzyme has no metal dependency | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 64000 | - |
1 * 64000, cytoplasmic enzyme, SDS-PAGE | Escherichia coli |
3.2.1.28 | 74000 | - |
gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.28 | trehalose + H2O | Escherichia coli | pathway of trehalose utilization | D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.28 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.28 | from an overexpressing strain, cytoplasmic enzyme | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.28 | trehalose + H2O | - |
Escherichia coli | D-glucose | - |
? | |
3.2.1.28 | trehalose + H2O | pathway of trehalose utilization | Escherichia coli | D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.28 | monomer | 1 * 64000, cytoplasmic enzyme, SDS-PAGE | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 6 | - |
- |
Escherichia coli |