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Literature summary extracted from

  • De Almeida, E.M.; de Lourdes Polizeli, M.; Terenzi, H.F.; Jorge, J.A.
    Biochemical characterization of a Ca2+-dependent acid trehalase activity from the thermophilic fungus Chaetomium thermophilum var. coprophilum (1999), FEMS Microbiol. Lett., 171, 11-15.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 ADP 1 mM, 53% inhibition Thermochaetoides thermophila
3.2.1.28 Al3+ 1 mM, complete inhibition Thermochaetoides thermophila
3.2.1.28 ATP 1 mM, 80% inhibition Thermochaetoides thermophila
3.2.1.28 barium ion 1 mM, complete inhibition Thermochaetoides thermophila
3.2.1.28 Cu2+ 1 mM, complete inhibition Thermochaetoides thermophila
3.2.1.28 EDTA 1 mM, 55% inhibition Thermochaetoides thermophila
3.2.1.28 Hg2+ 1 mM, complete inhibition Thermochaetoides thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 0.63
-
 trehalose presence of Ca2+ Thermochaetoides thermophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.28 Ca2+ stimulates Thermochaetoides thermophila
3.2.1.28 Co2+ stimulates Thermochaetoides thermophila
3.2.1.28 Mn2+ stimulates Thermochaetoides thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 98000
-
 2 * 98000, SDS-PAGE Thermochaetoides thermophila
3.2.1.28 200000
-
 gel filtration Thermochaetoides thermophila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Thermochaetoides thermophila
-
 var. coprophilum
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.28 no glycoprotein no carbohydrate detected Thermochaetoides thermophila

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28
-
 Thermochaetoides thermophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 120
-
-
 Thermochaetoides thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 trehalose + H2O
-
 Thermochaetoides thermophila D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 dimer 2 * 98000, SDS-PAGE Thermochaetoides thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 55
-
-
 Thermochaetoides thermophila

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.28 50 60 50°C: 39% of maximal activity, 60°C: 44% of maximal activity Thermochaetoides thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.28 additional information
-
 addition of Ca2+ decreases stability, half-life at 65°C is diminished about 2fold Thermochaetoides thermophila
3.2.1.28 50
-
 30 min, stable up to Thermochaetoides thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 6.5
-
-
 Thermochaetoides thermophila