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Literature summary extracted from

  • Kadowaki, M.K.; de Lourdes Polizeli, M.; Terenzi, H.F.; Jorge, J.A.
    Characterization of trehalase activities from the thermophilic fungus Scytalidium thermophilum (1996), Biochim. Biophys. Acta, 1291, 199-205.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.28 Ca2+ and Mn2+ protect from thermoinactivation Mycothermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 ADP
-
 Mycothermus thermophilus
3.2.1.28 Al3+ 1 mM AlCl3, complete inhibition Mycothermus thermophilus
3.2.1.28 ATP
-
 Mycothermus thermophilus
3.2.1.28 Cu2+ 1 mM CuSO4, complete inhibition of extracellular enzyme, 47% inhibition of intracellular enzyme Mycothermus thermophilus
3.2.1.28 EDTA 1 mM, 30% inhibition of extracellular enzyme, 54% inhibition of intracellular enzyme Mycothermus thermophilus
3.2.1.28 Hg2+ 1 mM HgCl2, complete inhibition Mycothermus thermophilus
3.2.1.28 NH4Cl 1 mM NH4Cl, 22% inhibition of extracellular enzyme, 27% inhibition of intracellular enzyme Mycothermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 2.24
-
 trehalose intracellular enzyme Mycothermus thermophilus
3.2.1.28 3.58
-
 trehalose extracellular enzyme Mycothermus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 extracellular intracellular and extracellular enzyme Mycothermus thermophilus
-
-
3.2.1.28 intracellular intracellular and extracellular enzyme Mycothermus thermophilus 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.28 Ca2+ activates Mycothermus thermophilus
3.2.1.28 Co2+ slight stimulation Mycothermus thermophilus
3.2.1.28 Mn2+ activates Mycothermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 82000
-
 5 * 82000, extracellular enzyme, SDS-PAGE Mycothermus thermophilus
3.2.1.28 85000
-
 5 * 85000, intracellular enzyme, SDS-PAGE Mycothermus thermophilus
3.2.1.28 370000
-
 extracellular enzyme, gel filtration Mycothermus thermophilus
3.2.1.28 398000
-
 intracellular enzyme, gel filtration Mycothermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Mycothermus thermophilus
-
 intracellular and extracellular threhalase
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.28 glycoprotein extracellular enzyme contains 81% carbohydrate, intracellular enzyme contains 51% carbohydrate Mycothermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 1775
-
 extracellular enzyme Mycothermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 trehalose + H2O absolute specificity Mycothermus thermophilus D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 pentamer 5 * 82000, extracellular enzyme, SDS-PAGE Mycothermus thermophilus
3.2.1.28 pentamer 5 * 85000, intracellular enzyme, SDS-PAGE Mycothermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 60
-
 extracellular enzyme Mycothermus thermophilus
3.2.1.28 65
-
 intracellular enzyme Mycothermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.28 60
-
 extracellular enzyme has a half-life of 3 min. Intracellular enzyme loses 20% of activity after 40 min when incubated without Ca2+, and is fully stable in presence of Ca2+ Mycothermus thermophilus
3.2.1.28 65
-
 intracellular enzyme has a half-life of 4.5 min when incubated without Ca2+ Mycothermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 6
-
 extracellular and intracellular enzyme Mycothermus thermophilus