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Literature summary extracted from
- Trehalase from the cellular slime mold Dictyostelium discoideum: purification and characterization of the homogeneous enzyme from myxamoebae (1983), Arch. Biochem. Biophys., 222, 561-573.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.28 | stable for at least 4 successive freeze-thaw cycles | Dictyostelium discoideum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.28 | Ca2+ | 1.0 mM CaCl2, 9% loss of activity | Dictyostelium discoideum |  |
| 3.2.1.28 | Cu2+ | 1.0 mM CuSO4, 13% loss of activity | Dictyostelium discoideum | |
| 3.2.1.28 | Hg2+ | 1 mM, 58% inhibition | Dictyostelium discoideum | |
| 3.2.1.28 | Mg2+ | 1.0 mM MgCl2, 13% loss of activity | Dictyostelium discoideum | |
| 3.2.1.28 | Tris | 100 mM, 28% inhibition | Dictyostelium discoideum | |
| 3.2.1.28 | Urea | 10 mM, 6% inhibition | Dictyostelium discoideum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.28 | 1.2 | - |
trehalose | - |
Dictyostelium discoideum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 95000 | - |
1 * 95000, SDS-PAGE | Dictyostelium discoideum |
| 3.2.1.28 | 100000 | - |
disc gel electrophoresis | Dictyostelium discoideum |
| 3.2.1.28 | 105000 | - |
gel filtration | Dictyostelium discoideum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.28 | Dictyostelium discoideum | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | glycoprotein | - |
Dictyostelium discoideum |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.28 | - |
Dictyostelium discoideum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 104 | - |
- |
Dictyostelium discoideum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.28 | -12°C, stable for at least 2 months | Dictyostelium discoideum |
| 3.2.1.28 | 4°-10°C, stable for at least 2-4 days | Dictyostelium discoideum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.28 | trehalose + H2O | absolute specificity | Dictyostelium discoideum | D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | monomer | 1 * 95000, SDS-PAGE | Dictyostelium discoideum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 50 | - |
- |
Dictyostelium discoideum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 45 | 50 | 5 min, stable | Dictyostelium discoideum |
| 3.2.1.28 | 55 | - |
5 min, 19% loss of activity | Dictyostelium discoideum |
| 3.2.1.28 | 60 | - |
5 min, 72% loss of activity | Dictyostelium discoideum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 5.5 | - |
- |
Dictyostelium discoideum |
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