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Literature summary extracted from

  • Sone, Y.; Misaki, A.
    Purification and characterization of beta-D-mannosidase and beta-N-acetyl-D-hexosaminidase of Tremella fuciformis (1978), J. Biochem., 83, 1135-1144.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.25 EDTA 11% stimulation at 5 mM Tremella fuciformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.25 Ag+ AgNO3 Tremella fuciformis
3.2.1.25 Ca2+ 5 mM Ca(NO3)2, 45% inhibition Tremella fuciformis
3.2.1.25 Cd2+ CdCl2 Tremella fuciformis
3.2.1.25 Hg2+ HgCl2 Tremella fuciformis
3.2.1.25 PCMB
-
 Tremella fuciformis
3.2.1.25 Zn2+ 10 mM ZnSO4, 30% inhibition Tremella fuciformis
3.2.1.52 Cd2+ 14%, 20% and 67% inhibition with 0.01 mM, 2 mM and 10 mM Tremella fuciformis
3.2.1.52 Hg2+ 37% inhibition with 0.01 mM, complete inhibition with 2 mM Tremella fuciformis
3.2.1.52 N-acetyl-D-glucosamine
-
 Tremella fuciformis
3.2.1.52 p-chloromercuribenzoate 43% inhibition with 0.5 mM Tremella fuciformis
3.2.1.52 Zn2+ 30%, 58% and 73% inhibition with 0.01 mM, 2 mM and 10 mM Tremella fuciformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.25 2.1
-
 p-nitrophenyl-beta-D-mannoside
-
 Tremella fuciformis
3.2.1.52 0.19
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Tremella fuciformis
3.2.1.52 0.31
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Tremella fuciformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.25 Ca2+ Ca(NO3)2, 22% stimulation at 0.5 mM Tremella fuciformis
3.2.1.25 MgSO4 26% stimulation at 10 mM Tremella fuciformis
3.2.1.25 ZnSO4 0.5 mM, 25% stimulation Tremella fuciformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.25 140000
-
 gel filtration Tremella fuciformis
3.2.1.52 125000
-
 gel filtration Tremella fuciformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Tremella fuciformis
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.25 Tremella fuciformis
-
-
-
3.2.1.52 Tremella fuciformis
-
-
-
3.2.1.52 Tremella fuciformis T-7
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.2.1.25 tends to be inactived by oxygen, dithiothreitol reactivates Tremella fuciformis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.25
-
 Tremella fuciformis
3.2.1.52 200fold to homogeneity Tremella fuciformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.25 20
-
-
 Tremella fuciformis
3.2.1.52 3.04
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Tremella fuciformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.25 beta1-4-mannobiose + H2O
-
 Tremella fuciformis ?
-
 ?
3.2.1.25 beta1-4-mannobiose + H2O
-
 Tremella fuciformis T-7 ?
-
 ?
3.2.1.25 Man-(beta1-4)GlcNAc + H2O
-
 Tremella fuciformis mannose + GlcNAc
-
 ?
3.2.1.25 Man-(beta1-4)GlcNAc + H2O
-
 Tremella fuciformis T-7 mannose + GlcNAc
-
 ?
3.2.1.25 mannotriose + H2O beta1-4-D-mannotriose Tremella fuciformis ?
-
 ?
3.2.1.25 mannotriose + H2O beta1-4-D-mannotriose Tremella fuciformis T-7 ?
-
 ?
3.2.1.25 p-nitrophenyl-beta-D-mannoside + H2O
-
 Tremella fuciformis p-nitrophenol + alpha-D-mannose
-
 ?
3.2.1.25 p-nitrophenyl-beta-D-mannoside + H2O
-
 Tremella fuciformis T-7 p-nitrophenol + alpha-D-mannose
-
 ?
3.2.1.52 beta-glucosaminyl-muramyl-peptide + H2O isolated from bacterial cell-wall peptidoglycan Tremella fuciformis N-acetyl-D-glucosamine + muramyl-peptide
-
 ?
3.2.1.52 N,N',N'',N'''-tetraacetylchitotetraose + 3 H2O
-
 Tremella fuciformis 4 N-acetyl-D-glucosamine
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Tremella fuciformis N-acetyl-D-glucosamine
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Tremella fuciformis N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Tremella fuciformis 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Tremella fuciformis 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Tremella fuciformis T-7 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 tri-N-acetylchitotriose + H2O
-
 Tremella fuciformis N-acetyl-D-glucosamine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.25 5
-
-
 Tremella fuciformis
3.2.1.52 5
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Tremella fuciformis
3.2.1.52 5
-
 substrate: N,N-diacetylchitobiose Tremella fuciformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.25 3.5
-
 rapid loss of activity Tremella fuciformis
3.2.1.25 5 5.5 37°C, 1 h, stable Tremella fuciformis
3.2.1.52 7
-
 stable around pH 7.0 Tremella fuciformis