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Literature summary extracted from

  • Muramatsu, T.; Egami, F.
    alpha-Mannosidase and beta-mannosidase from the liver of Turbo cortunus: Purification, properties and application in carbohydrate research (1967), J. Biochem., 62, 700-709.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.24 NaCl 50% activation up to 1.5 M Turbo cornutus

Application

EC Number Application Comment Organism
3.2.1.25 analysis application in carbohydrate research Turbo cornutus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 cysteine 31% inhibition at 0.02 mM Turbo cornutus
3.2.1.25 Cu2+ 0.2 mM, 76% inhibition Turbo cornutus
3.2.1.25 monoiodoacetate
-
 Turbo cornutus
3.2.1.25 Zn2+ 0.1 mM, 35% inhibition Turbo cornutus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 2.3
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Turbo cornutus
3.2.1.25 7.1
-
 phenyl beta-D-mannoside
-
 Turbo cornutus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.24 Zn2+ slight activation at 0.02 mM Turbo cornutus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 glycopeptide + H2O Turbo cornutus from ovalbumin alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 mannosyl rhamnose + H2O Turbo cornutus part of the repeating oligosaccharide unit of the O-antigenic lipopolysaccharide in Salmonella typhimurium alpha-D-mannopyranose + rhamnose
-
 ?
3.2.1.24 TAKA-amylase A + H2O Turbo cornutus
-
 alpha-D-mannopyranose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Turbo cornutus
-
-
-
3.2.1.25 Turbo cornutus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 near homogeneity, chromatography steps Turbo cornutus
3.2.1.25
-
 Turbo cornutus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 liver
-
 Turbo cornutus
-
3.2.1.25 liver
-
 Turbo cornutus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.25 additional information
-
-
 Turbo cornutus

Storage Stability

EC Number Storage Stability Organism
3.2.1.25 -20°C, stable for 2 or 3 months Turbo cornutus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 glycopeptide + H2O from ovalbumin Turbo cornutus alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 mannosyl rhamnose + H2O part of the repeating oligosaccharide unit of the O-antigenic lipopolysaccharide in Salmonella typhimurium Turbo cornutus alpha-D-mannopyranose + rhamnose
-
 ?
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Turbo cornutus p-nitrophenol + alpha-D-mannopyranose
-
 ?
3.2.1.24 TAKA-amylase A + H2O
-
 Turbo cornutus alpha-D-mannopyranose + ?
-
 ?
3.2.1.25 phenyl beta-D-mannoside + H2O
-
 Turbo cornutus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.24 -20
-
 no loss of activity within 3 months Turbo cornutus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 4
-
-
 Turbo cornutus
3.2.1.25 4
-
-
 Turbo cornutus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.25 3.2 5.2 pH 3.2: about 70% of maximal activity, pH 5.2: about 80% of maximal activity Turbo cornutus