BRENDA - Enzyme Database

Purification and characterization of a phospholipid-dependent alpha-mannosidase from rabbit liver

Forsee, W.T.; Schutzbach, J.S.; J. Biol. Chem. 256, 6577-6582 (1981)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.24
phosphatidylcholine
10fold stimulation at 0.002%
Oryctolagus cuniculus
3.2.1.24
phosphatidylethanolamine
8fold stimulation at 0.002%
Oryctolagus cuniculus
3.2.1.24
Triton X-100
optimal stimulation at 0.02%
Oryctolagus cuniculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.24
alpha-D-1,2-mannopyranosyl-mannopyranose
50% inhibition at 4 mM
Oryctolagus cuniculus
3.2.1.24
alpha-D-1,3-mannopyranosyl-alpha-D-mannopyranose
50% inhibition at 18 mM
Oryctolagus cuniculus
3.2.1.24
alpha-D-1,6-mannopyranosyl-mannopyranose
50% inhibition at 100 mM
Oryctolagus cuniculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.24
microsome
-
Oryctolagus cuniculus
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.24
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNac-betaGlcNAc + H2O
Oryctolagus cuniculus
-
alpha-D-mannopyranose + ?
-
Oryctolagus cuniculus
?
3.2.1.24
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manbeta(1-6)]ManbetaGlcNAc-beta-GlcNAc + H2O
Oryctolagus cuniculus
-
alpha-D-mannopyranose + ?
-
Oryctolagus cuniculus
?
3.2.1.24
Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
Oryctolagus cuniculus
-
alpha-D-mannopyranose + ?
-
Oryctolagus cuniculus
?
3.2.1.24
Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
Oryctolagus cuniculus
-
alpha-D-mannopyranose + ?
-
Oryctolagus cuniculus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.24
Oryctolagus cuniculus
-
rabbit
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.24
near homogeneity, chromatography steps
Oryctolagus cuniculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.24
liver
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.24
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNac-betaGlcNAc + H2O
-
136002
Oryctolagus cuniculus
alpha-D-mannopyranose + ?
-
136002
Oryctolagus cuniculus
?
3.2.1.24
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manbeta(1-6)]ManbetaGlcNAc-beta-GlcNAc + H2O
-
136002
Oryctolagus cuniculus
alpha-D-mannopyranose + ?
-
136002
Oryctolagus cuniculus
?
3.2.1.24
Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
-
136002
Oryctolagus cuniculus
alpha-D-mannopyranose + ?
-
136002
Oryctolagus cuniculus
?
3.2.1.24
Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
-
136002
Oryctolagus cuniculus
alpha-D-mannopyranose + ?
-
136002
Oryctolagus cuniculus
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.24
4
-
alpha-1,2-mannopyranosyl-mannopyranose
-
Oryctolagus cuniculus
3.2.1.24
18
-
alpha-1,3-mannopyranosyl-mannopyranose
-
Oryctolagus cuniculus
3.2.1.24
100
-
alpha-1,6-mannopyranosyl-mannopyranose
-
Oryctolagus cuniculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.24
phosphatidylcholine
10fold stimulation at 0.002%
Oryctolagus cuniculus
3.2.1.24
phosphatidylethanolamine
8fold stimulation at 0.002%
Oryctolagus cuniculus
3.2.1.24
Triton X-100
optimal stimulation at 0.02%
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.24
alpha-D-1,2-mannopyranosyl-mannopyranose
50% inhibition at 4 mM
Oryctolagus cuniculus
3.2.1.24
alpha-D-1,3-mannopyranosyl-alpha-D-mannopyranose
50% inhibition at 18 mM
Oryctolagus cuniculus
3.2.1.24
alpha-D-1,6-mannopyranosyl-mannopyranose
50% inhibition at 100 mM
Oryctolagus cuniculus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.24
4
-
alpha-1,2-mannopyranosyl-mannopyranose
-
Oryctolagus cuniculus
3.2.1.24
18
-
alpha-1,3-mannopyranosyl-mannopyranose
-
Oryctolagus cuniculus
3.2.1.24
100
-
alpha-1,6-mannopyranosyl-mannopyranose
-
Oryctolagus cuniculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.24
microsome
-
Oryctolagus cuniculus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.24
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNac-betaGlcNAc + H2O
Oryctolagus cuniculus
-
alpha-D-mannopyranose + ?
-
Oryctolagus cuniculus
?
3.2.1.24
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manbeta(1-6)]ManbetaGlcNAc-beta-GlcNAc + H2O
Oryctolagus cuniculus
-
alpha-D-mannopyranose + ?
-
Oryctolagus cuniculus
?
3.2.1.24
Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
Oryctolagus cuniculus
-
alpha-D-mannopyranose + ?
-
Oryctolagus cuniculus
?
3.2.1.24
Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
Oryctolagus cuniculus
-
alpha-D-mannopyranose + ?
-
Oryctolagus cuniculus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.24
near homogeneity, chromatography steps
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.24
liver
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.24
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNac-betaGlcNAc + H2O
-
136002
Oryctolagus cuniculus
alpha-D-mannopyranose + ?
-
136002
Oryctolagus cuniculus
?
3.2.1.24
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manbeta(1-6)]ManbetaGlcNAc-beta-GlcNAc + H2O
-
136002
Oryctolagus cuniculus
alpha-D-mannopyranose + ?
-
136002
Oryctolagus cuniculus
?
3.2.1.24
Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
-
136002
Oryctolagus cuniculus
alpha-D-mannopyranose + ?
-
136002
Oryctolagus cuniculus
?
3.2.1.24
Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
-
136002
Oryctolagus cuniculus
alpha-D-mannopyranose + ?
-
136002
Oryctolagus cuniculus
?