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Literature summary extracted from

  • Forsee, W.T.; Schutzbach, J.S.
    Purification and characterization of a phospholipid-dependent alpha-mannosidase from rabbit liver (1981), J. Biol. Chem., 256, 6577-6582.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.24 phosphatidylcholine 10fold stimulation at 0.002% Oryctolagus cuniculus
3.2.1.24 phosphatidylethanolamine 8fold stimulation at 0.002% Oryctolagus cuniculus
3.2.1.24 Triton X-100 optimal stimulation at 0.02% Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 alpha-D-1,2-mannopyranosyl-mannopyranose 50% inhibition at 4 mM Oryctolagus cuniculus
3.2.1.24 alpha-D-1,3-mannopyranosyl-alpha-D-mannopyranose 50% inhibition at 18 mM Oryctolagus cuniculus
3.2.1.24 alpha-D-1,6-mannopyranosyl-mannopyranose 50% inhibition at 100 mM Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.24 microsome
-
 Oryctolagus cuniculus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNac-betaGlcNAc + H2O Oryctolagus cuniculus
-
 alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manbeta(1-6)]ManbetaGlcNAc-beta-GlcNAc + H2O Oryctolagus cuniculus
-
 alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O Oryctolagus cuniculus
-
 alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O Oryctolagus cuniculus
-
 alpha-D-mannopyranose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Oryctolagus cuniculus
-
 rabbit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 near homogeneity, chromatography steps Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 liver
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNac-betaGlcNAc + H2O
-
 Oryctolagus cuniculus alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manbeta(1-6)]ManbetaGlcNAc-beta-GlcNAc + H2O
-
 Oryctolagus cuniculus alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
-
 Oryctolagus cuniculus alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
-
 Oryctolagus cuniculus alpha-D-mannopyranose + ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.24 4
-
 alpha-1,2-mannopyranosyl-mannopyranose
-
 Oryctolagus cuniculus
3.2.1.24 18
-
 alpha-1,3-mannopyranosyl-mannopyranose
-
 Oryctolagus cuniculus
3.2.1.24 100
-
 alpha-1,6-mannopyranosyl-mannopyranose
-
 Oryctolagus cuniculus